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Improved detection of respiratory viruses in pediatric outpatients with acute respiratory illness by real-time PCR using nasopharyngeal flocked swabs
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Munywoki, Patrick, Hamid, Fauzat, Mutunga, Martin, Welch, Steve, Cane, Patricia and Nokes, D. James (2011) Improved detection of respiratory viruses in pediatric outpatients with acute respiratory illness by real-time PCR using nasopharyngeal flocked swabs. Journal of Clinical Microbiology, Vol.49 (No.9). pp. 3365-3367. doi:10.1128/JCM.02231-10 ISSN 0095-1137.
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WRAP_Nokes_0380313-lf-101111-munywoki_et_al.pdf - Accepted Version - Requires a PDF viewer. Download (108Kb) |
Official URL: http://dx.doi.org/10.1128/JCM.02231-10
Abstract
Detection of respiratory viruses by realtime multiplexed PCR (M-PCR) and of RSV by M-PCR and immunofluorescence(IF) was evaluated using specimens collected by
nasopharyngeal flocked swab(NFS) and nasal wash(NW). In children with mild respiratory
illness, NFS collection was superior to NW collection for detection of viruses by M-PCR
(sensitivity 89.6% vs 79.2%, P=0.0043). NFS collection was non-inferior to NW collection in
detecting RSV by IF.
Item Type: | Journal Article | ||||
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Subjects: | R Medicine > RC Internal medicine R Medicine > RJ Pediatrics |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Respiratory infections in children -- Diagnosis, Polymerase chain reaction, Diagnostic specimens -- Methodology | ||||
Journal or Publication Title: | Journal of Clinical Microbiology | ||||
Publisher: | American Society for Microbiology | ||||
ISSN: | 0095-1137 | ||||
Official Date: | 20 July 2011 | ||||
Dates: |
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Volume: | Vol.49 | ||||
Number: | No.9 | ||||
Page Range: | pp. 3365-3367 | ||||
DOI: | 10.1128/JCM.02231-10 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Date of first compliant deposit: | 18 December 2015 | ||||
Date of first compliant Open Access: | 18 December 2015 | ||||
Funder: | Bill & Melinda Gates Foundation, Wellcome Trust (London, England) | ||||
Grant number: | 076278 (WT), 090853 (WT) |
Data sourced from Thomson Reuters' Web of Knowledge
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