Munywoki, Patrick, Hamid, Fauzat, Mutunga, Martin, Welch, Steve, Cane, Patricia and Nokes, D. James. (2011) Improved detection of respiratory viruses in pediatric outpatients with acute respiratory illness by real-time PCR using nasopharyngeal flocked swabs. Journal of Clinical Microbiology, Vol.49 (No.9). pp. 3365-3367. ISSN 0095-1137

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Abstract

Detection of respiratory viruses by realtime multiplexed PCR (M-PCR) and of RSV by M-PCR and immunofluorescence(IF) was evaluated using specimens collected by nasopharyngeal flocked swab(NFS) and nasal wash(NW). In children with mild respiratory illness, NFS collection was superior to NW collection for detection of viruses by M-PCR (sensitivity 89.6% vs 79.2%, P=0.0043). NFS collection was non-inferior to NW collection in detecting RSV by IF.

Item Type:	Journal Article
Subjects:	R Medicine > RC Internal medicine R Medicine > RJ Pediatrics
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Respiratory infections in children -- Diagnosis, Polymerase chain reaction, Diagnostic specimens -- Methodology
Journal or Publication Title:	Journal of Clinical Microbiology
Publisher:	American Society for Microbiology
ISSN:	0095-1137
Date:	20 July 2011
Volume:	Vol.49
Number:	No.9
Page Range:	pp. 3365-3367
Identification Number:	10.1128/JCM.02231-10
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Bill & Melinda Gates Foundation, Wellcome Trust (London, England)
Grant number:	076278 (WT), 090853 (WT)
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URI:	http://wrap.warwick.ac.uk/id/eprint/39441

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