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An Exo2 derivative affects ER and Golgi morphology and vacuolar sorting in a tissue-specific manner in arabidopsis
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Sorieul, Mathias, Langhans, Markus, Guetzoyan, Lucie J., Hillmer, Stefan, 1959-, Clarkson, Guy J., Lord, Mike (J. Mike), Roberts, L. M. (Lynne M.), Robinson, David G., Spooner, Robert A. and Frigerio, Lorenzo. (2011) An Exo2 derivative affects ER and Golgi morphology and vacuolar sorting in a tissue-specific manner in arabidopsis. Traffic, Vol.12 (No.11). pp. 1552-1562. ISSN 13989219
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Official URL: http://dx.doi.org/10.1111/j.1600-0854.2011.01258.x
Abstract
We screened a panel of compounds derived from Exo2—a drug that perturbs post-Golgi compartments and trafficking in mammalian cells—for their effect on the secretory pathway in Arabidopsis root epidermal cells. While Exo2 and most related compounds had no significant effect, one Exo2 derivative, named LG8, induced severe morphological alterations in both the Golgi (at high concentrations) and the endoplasmic reticulum (ER). LG8 causes the ER to form foci of interconnecting tubules, which at the ultrastructural level appear similar to those previously reported in Arabidopsis roots after treatment with the herbicide oryzalin. In cotyledonary leaves, LG8 causes redistribution of a trans Golgi network (TGN) marker to the vacuole. LG8 affects the anterograde secretory pathway by inducing secretion of vacuolar cargo and preventing the brassinosteroid receptor BRI1 from reaching the plasma membrane. Uptake and arrival at the TGN of the endocytic marker FM4-64 is not affected. Unlike the ADP ribosylation factor-GTP exchange factor (ARF-GEF) inhibitor brefeldin A (BFA), LG8 affects these post-Golgi events without causing the formation of BFA bodies. Up to concentrations of 50 µm, the effects of LG8 are reversible.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics |
| Divisions: | Faculty of Science > Life Sciences (2010- ) |
| Library of Congress Subject Headings (LCSH): | Golgi apparatus, Chemogenomics, Endocytosis, Endoplasmic reticulum, Endosomes, Plant vacuoles, Arabidopsis |
| Journal or Publication Title: | Traffic |
| Publisher: | Wiley-Blackwell Publishing Ltd. |
| ISSN: | 13989219 |
| Date: | 24 August 2011 |
| Volume: | Vol.12 |
| Number: | No.11 |
| Page Range: | pp. 1552-1562 |
| Identification Number: | 10.1111/j.1600-0854.2011.01258.x |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| URI: | http://wrap.warwick.ac.uk/id/eprint/39532 |
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