Pronephric Tubulogenesis Requires Daam1-Mediated Planar Cell Polarity Signaling
Miller, R. K., Gomez de la Torre Canny, S., Jang, C.-W., Cho, K., Ji, H., Wagner, D. S., Jones, E. A. (Elizabeth A.), Habas, R. and McCrea, P. D.. (2011) Pronephric Tubulogenesis Requires Daam1-Mediated Planar Cell Polarity Signaling. Journal of the American Society of Nephrology, Vol.22 (No.9). pp. 1654-1664. ISSN 1046-6673Full text not available from this repository.
Official URL: http://dx.doi.org/10.1681/ASN.2010101086
Canonical beta-catenin-mediated Wnt signaling is essential for the induction of nephron development. Noncanonical Wnt/planar cell polarity (PCP) pathways contribute to processes such as cell polarization and cytoskeletal modulation in several tissues. Although PCP components likely establish the plane of polarization in kidney tubulogenesis, whether PCP effectors directly modulate the actin cytoskeleton in tubulogenesis is unknown. Here, we investigated the roles of Wnt PCP components in cytoskeletal assembly during kidney tubule morphogenesis in Xenopus laevis and zebrafish. We found that during tubulogenesis, the developing pronephric anlagen expresses Daam1 and its interacting Rho-GEF (WGEF), which compose one PCP/noncanonical Wnt pathway branch. Knockdown of Daam1 resulted in reduced expression of late pronephric epithelial markers with no apparent effect upon early markers of patterning and determination. Inhibiting various points in the Daam1 signaling pathway significantly reduced pronephric tubulogenesis. These data indicate that pronephric tubulogenesis requires the Daam1/WGEF/Rho PCP pathway.
|Item Type:||Journal Article|
|Subjects:||Q Science > Q Science (General)|
|Divisions:||Faculty of Science > Life Sciences (2010- )|
|Journal or Publication Title:||Journal of the American Society of Nephrology|
|Publisher:||American Society of Nephrology|
|Number of Pages:||11|
|Page Range:||pp. 1654-1664|
|Access rights to Published version:||Restricted or Subscription Access|
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