Rahman, Md Tanvir , Crombie, Andrew, Chen, Yin, Stralis-Pavese, Nancy, Bodrossy, Levente, Meir, Patrick, McNamara, Niall P and Murrell, J Colin. (2011) Environmental distribution and abundance of the facultative methanotroph Methylocella. ISME Journal, Vol.5 (No.6). pp. 1061-1066. ISSN 1751-7362

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Abstract

Methylocella spp. are facultative methanotrophs, which are able to grow not only on methane but also on multicarbon substrates such as acetate, pyruvate or malate. Methylocella spp. were previously thought to be restricted to acidic soils such as peatlands, in which they may have a key role in methane oxidation. There is little information on the abundance and distribution of Methylocella spp. in the environment. New primers were designed, and a real-time quantitative PCR method was developed and validated targeting Methylocella mmoX (encoding the alpha-subunit of the soluble methane monooxygenase) that allowed the quantification of Methylocella spp. in environmental samples. We also developed and validated specific PCR assays, which target 16S rRNA genes of known Methylocella spp. These were used to investigate the distribution of Methylocella spp. in a variety of environmental samples. It was revealed that Methylocella species are widely distributed in nature and not restricted to acidic environments. The ISME Journal (2011) 5, 1061-1066; doi:10.1038/ismej.2010.190; published online 16 December 2010 Subject Category: microbial ecology and functional diversity of natural habitats

Item Type:	Journal Article
Subjects:	Q Science > Q Science (General)
Divisions:	Faculty of Science > Life Sciences (2010- )
Journal or Publication Title:	ISME Journal
Publisher:	Nature Publishing Group
ISSN:	1751-7362
Official Date:	2011
Volume:	Vol.5
Number:	No.6
Number of Pages:	6
Page Range:	pp. 1061-1066
Identification Number:	10.1038/ismej.2010.190
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
URI:	http://wrap.warwick.ac.uk/id/eprint/39583

Data sourced from Thomson Reuters' Web of Knowledge

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