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Active Autotrophic Ammonia-Oxidizing Bacteria in Biofilm Enrichments from Simulated Creek Ecosystems at Two Ammonium Concentrations Respond to Temperature Manipulation
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Avrahami, S., Jia, Z., Neufeld, J. D., Murrell, J. C. (J. Colin), Conrad, R. and Kusel, K. (2011) Active Autotrophic Ammonia-Oxidizing Bacteria in Biofilm Enrichments from Simulated Creek Ecosystems at Two Ammonium Concentrations Respond to Temperature Manipulation. Applied and Environmental Microbiology, Vol.77 (No.20). pp. 7329-7338. doi:10.1128/AEM.05864-11
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Official URL: http://dx.doi.org/10.1128/AEM.05864-11
Abstract
The first step of nitrification, the oxidation of ammonia to nitrite, is important for reducing eutrophication in freshwater environments when coupled with anammox (anaerobic ammonium oxidation) or denitrification. We analyzed active formerly biofilm-associated aerobic ammonia-oxidizing communities originating from Ammerbach (AS) and Leutra South (LS) stream water (683 +/- 550 [ mean +/- standard deviation] and 16 +/- 7 mu M NH(4)(+), respectively) that were developed in a flow-channel experiment and incubated under three temperature regimens. By stable-isotope probing using (13)CO(2), we found that members of the Bacteria and not Archaea were the functionally dominant autotrophic ammonia oxidizers at all temperatures under relatively high ammonium loads. The copy numbers of bacterial amoA genes in (13)C-labeled DNA were lower at 30 degrees C than at 13 degrees C in both stream enrichment cultures. However, the community composition of the ammonia-oxidizing bacteria (AOB) in the 13C-labeled DNA responded differently to temperature manipulation at two ammonium concentrations. In LS enrichments incubated at the in situ temperature (13 degrees C), Nitrosomonas oligotropha-like sequences were retrieved with sequences from Nitrosospira AmoA cluster 4, while the proportion of Nitrosospira sequences increased at higher temperatures. In AS enrichments incubated at 13 degrees C and 20 degrees C, AmoA cluster 4 sequences were dominant; Nitrosomonas nitrosa-like sequences dominated at 30 degrees C. Biofilm-associated AOB communities were affected differentially by temperature at two relatively high ammonium concentrations, implicating them in a potential role in governing contaminated freshwater AOB distributions.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > Q Science (General) | ||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Applied and Environmental Microbiology | ||||
| Publisher: | American Society for Microbiology | ||||
| ISSN: | 0099-2240 | ||||
| Official Date: | 2011 | ||||
| Dates: |
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| Volume: | Vol.77 | ||||
| Number: | No.20 | ||||
| Page Range: | pp. 7329-7338 | ||||
| DOI: | 10.1128/AEM.05864-11 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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