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Ghrelin promotes nuclear factor kappa-B activation in a human B-lymphocyte cell line

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Sung, E. Z. H., Silva, N. F., Goodyear, S. J., McTernan, P. G. (Philip G.), Arasaradnam, Ramesh P. and Nwokolo, Chuka U. (2011) Ghrelin promotes nuclear factor kappa-B activation in a human B-lymphocyte cell line. Molecular Biology Reports, Vol.38 (No.8). pp. 4833-4838. doi:10.1007/s11033-010-0617-x

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Official URL: http://dx.doi.org/10.1007/s11033-010-0617-x

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Abstract

Ghrelin, an orexigenic hormone of gastric origin that stimulates growth hormone secretion, may modulate inflammation. This experimental study examines the effect of ghrelin on NF kappa B (p65 subunit), a transcriptional factor involved in inflammation on a human B-lymphocyte cell (WILCL). After confirming the expression of ghrelin receptor protein using western blotting the cells were transferred to wells maintaining a density of 1 x 10(6) cells per ml and a proportion activated with phytohaemagluttinin. Activated and resting cells were exposed to octanoyl-, desoctanoyl ghrelin and a non-peptide ghrelin agonist (Pfizer CP-464709) in increasing concentrations for 6 h. Cell protein extracts were analyzed for NF kappa B activation using Trans AM NF kappa B p65 assay. IL-6, IL-8, IL-10, IL-13 and TNF alpha were measured in the media using Lincoplex human cytokine assay. In octanoyl ghrelin treated resting cells, NF kappa B activity (Optical Density OD(450nm)) (mean +/- A SEM) in control cells was 0.42 +/- A 0.10 and increased to 0.61 +/- A 0.20 (P = 0.044), 0.54 +/- A 0.10 (P = 0.043), 0.52 +/- A 0.08 at 1, 10 and 100 nM concentrations respectively. No effect was detected with desoctanoyl ghrelin or ghrelin agonist and no specific change in cytokine production. In conclusion, Octanoyl ghrelin increased NF kappa B activation by up to 50% in a B-lymphocyte cell line suggesting an effect on the inflammatory process.

Item Type: Journal Article
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016)
Faculty of Medicine > Warwick Medical School
Journal or Publication Title: Molecular Biology Reports
Publisher: Springer Netherlands
ISSN: 0301-4851
Official Date: November 2011
Dates:
DateEvent
November 2011Published
Volume: Vol.38
Number: No.8
Number of Pages: 6
Page Range: pp. 4833-4838
DOI: 10.1007/s11033-010-0617-x
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

Data sourced from Thomson Reuters' Web of Knowledge

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