Ghrelin promotes nuclear factor kappa-B activation in a human B-lymphocyte cell line
Sung, E. Z. H., Silva, N. F., Goodyear, S. J., McTernan, P. G. (Philip G.), Arasaradnam, Ramesh P. and Nwokolo, Chuka U.. (2011) Ghrelin promotes nuclear factor kappa-B activation in a human B-lymphocyte cell line. Molecular Biology Reports, Vol.38 (No.8). pp. 4833-4838. ISSN 0301-4851Full text not available from this repository.
Official URL: http://dx.doi.org/10.1007/s11033-010-0617-x
Ghrelin, an orexigenic hormone of gastric origin that stimulates growth hormone secretion, may modulate inflammation. This experimental study examines the effect of ghrelin on NF kappa B (p65 subunit), a transcriptional factor involved in inflammation on a human B-lymphocyte cell (WILCL). After confirming the expression of ghrelin receptor protein using western blotting the cells were transferred to wells maintaining a density of 1 x 10(6) cells per ml and a proportion activated with phytohaemagluttinin. Activated and resting cells were exposed to octanoyl-, desoctanoyl ghrelin and a non-peptide ghrelin agonist (Pfizer CP-464709) in increasing concentrations for 6 h. Cell protein extracts were analyzed for NF kappa B activation using Trans AM NF kappa B p65 assay. IL-6, IL-8, IL-10, IL-13 and TNF alpha were measured in the media using Lincoplex human cytokine assay. In octanoyl ghrelin treated resting cells, NF kappa B activity (Optical Density OD(450nm)) (mean +/- A SEM) in control cells was 0.42 +/- A 0.10 and increased to 0.61 +/- A 0.20 (P = 0.044), 0.54 +/- A 0.10 (P = 0.043), 0.52 +/- A 0.08 at 1, 10 and 100 nM concentrations respectively. No effect was detected with desoctanoyl ghrelin or ghrelin agonist and no specific change in cytokine production. In conclusion, Octanoyl ghrelin increased NF kappa B activation by up to 50% in a B-lymphocyte cell line suggesting an effect on the inflammatory process.
|Item Type:||Journal Article|
|Subjects:||R Medicine > R Medicine (General)|
|Divisions:||Faculty of Medicine > Warwick Medical School > Metabolic and Vascular Health
Faculty of Medicine > Warwick Medical School
|Journal or Publication Title:||Molecular Biology Reports|
|Number of Pages:||6|
|Page Range:||pp. 4833-4838|
|Access rights to Published version:||Restricted or Subscription Access|
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