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Ghrelin promotes nuclear factor kappa-B activation in a human B-lymphocyte cell line
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Sung, E. Z. H., Silva, N. F., Goodyear, S. J., McTernan, P. G. (Philip G.), Arasaradnam, Ramesh P. and Nwokolo, Chuka U.. (2011) Ghrelin promotes nuclear factor kappa-B activation in a human B-lymphocyte cell line. Molecular Biology Reports, Vol.38 (No.8). pp. 4833-4838. ISSN 0301-4851
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Official URL: http://dx.doi.org/10.1007/s11033-010-0617-x
Abstract
Ghrelin, an orexigenic hormone of gastric origin that stimulates growth hormone secretion, may modulate inflammation. This experimental study examines the effect of ghrelin on NF kappa B (p65 subunit), a transcriptional factor involved in inflammation on a human B-lymphocyte cell (WILCL). After confirming the expression of ghrelin receptor protein using western blotting the cells were transferred to wells maintaining a density of 1 x 10(6) cells per ml and a proportion activated with phytohaemagluttinin. Activated and resting cells were exposed to octanoyl-, desoctanoyl ghrelin and a non-peptide ghrelin agonist (Pfizer CP-464709) in increasing concentrations for 6 h. Cell protein extracts were analyzed for NF kappa B activation using Trans AM NF kappa B p65 assay. IL-6, IL-8, IL-10, IL-13 and TNF alpha were measured in the media using Lincoplex human cytokine assay. In octanoyl ghrelin treated resting cells, NF kappa B activity (Optical Density OD(450nm)) (mean +/- A SEM) in control cells was 0.42 +/- A 0.10 and increased to 0.61 +/- A 0.20 (P = 0.044), 0.54 +/- A 0.10 (P = 0.043), 0.52 +/- A 0.08 at 1, 10 and 100 nM concentrations respectively. No effect was detected with desoctanoyl ghrelin or ghrelin agonist and no specific change in cytokine production. In conclusion, Octanoyl ghrelin increased NF kappa B activation by up to 50% in a B-lymphocyte cell line suggesting an effect on the inflammatory process.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | R Medicine > R Medicine (General) | ||||
| Divisions: | Faculty of Medicine > Warwick Medical School > Translational & Systems Medicine > Metabolic and Vascular Health Faculty of Medicine > Warwick Medical School |
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| Journal or Publication Title: | Molecular Biology Reports | ||||
| Publisher: | Springer Netherlands | ||||
| ISSN: | 0301-4851 | ||||
| Official Date: | November 2011 | ||||
| Dates: |
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| Volume: | Vol.38 | ||||
| Number: | No.8 | ||||
| Number of Pages: | 6 | ||||
| Page Range: | pp. 4833-4838 | ||||
| Identification Number: | 10.1007/s11033-010-0617-x | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| URI: | http://wrap.warwick.ac.uk/id/eprint/39656 |
Data sourced from Thomson Reuters' Web of Knowledge
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