Grimaldi, Giulia, Christian, Mark, Steel, Jennifer H., Henriet, Patrick, Poutanen, Matti and Brosens, Jan J. (2011) Down-regulation of the histone methyltransferase EZH2 contributes to the epigenetic programming of decidualizing human endometrial stromal cells. Molecular Endocrinology, Vol.25 (No.11). pp. 1892-1903. doi:10.1210/me.2011-1139

Preview	Text WRAP_Brosens_Downregulation_histone_EZH2FINAL.pdf - Accepted Version Download (315Kb)
	Text (Figures) WRAP_Brosens_FiguresEZH2.pdf - Supplemental Material Embargoed item. Restricted access to Repository staff only Download (13Mb)

Request Changes to record.

Abstract

Differentiation of human endometrial stromal cells (HESC) into decidual cells represents a highly coordinated process essential for embryo implantation. We show that decidualizing HESC down-regulate the histone methyltransferase enhancer of Zeste homolog 2 (EZH2), resulting in declining levels of trimethylation of histone 3 on lysine 27 (H3K27me3) at the proximal promoters of key decidual marker genes PRL and IGFBP1. Loss of H3K27me3 was associated with a reciprocal enrichment in acetylation of the same lysine residue, indicating active remodeling from repressive to transcriptionally permissive chromatin. Chromatin immunoprecipitation coupled with DNA microarray analysis demonstrated that decidualization triggers genome-wide changes in H3K27me3 distribution that only partly overlap those observed upon EZH2 knockdown in undifferentiated HESC. Gene ontology revealed that gain of the repressive H3K27me3 mark in response to decidualization and upon EZH2 knockdown in undifferentiated cells was enriched at the promoter regions of genes involved in transcriptional regulation and growth/cell proliferation, respectively. However, loss of the H3K27me3 mark (indicating increased chromatin accessibility) in decidualizing cells and upon EZH2 knockdown occurred at selective loci enriched for genes functionally implicated in responses to stimulus. In agreement, EZH2 knockdown in undifferentiated HESC was sufficient to augment the induction of decidual marker genes in response to cyclic AMP and progesterone signaling. Thus, loss of EZH2-dependent methyltransferase activity in the endometrium is integral to the process of chromatin remodeling that enables the transition from a proliferative to a decidual phenotype in response to differentiation cues.

Item Type:	Journal Article
Subjects:	Q Science > QP Physiology
Divisions:	Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Conception -- Physiology, Endometrium -- Physiology, Decidua
Journal or Publication Title:	Molecular Endocrinology
Publisher:	Endocrine Society
ISSN:	0888-8809
Official Date:	November 2011
Dates:	Date Event November 2011 Published
Date of first compliant deposit:	18 December 2015
Volume:	Vol.25
Number:	No.11
Page Range:	pp. 1892-1903
DOI:	10.1210/me.2011-1139
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Genesis Research Trust, National Institute for Health Research (Great Britain) (NIHR)

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Repository staff actions (login required)

View Item