Multivesicular bodies mature from the trans-golgi network/early endosome in Arabidopsis
Scheuring, David, Viotti, Corrado, Kruger, Falco, Kunzl, Fabian, Sturm, Silke, Bubeck, Julia, Hillmer, Stefan, 1959-, Frigerio, Lorenzo, Robinson, David G., Pimpl, Peter and Schumacher, Karin. (2011) Multivesicular bodies mature from the trans-golgi network/early endosome in Arabidopsis. Plant Cell, Vol.23 (No.9). pp. 3463-3481. ISSN 1040-4651Full text not available from this repository.
Official URL: http://dx.doi.org/10.1105/tpc.111.086918
The plant trans-Golgi network/early endosome (TGN/EE) is a major hub for secretory and endocytic trafficking with complex molecular mechanisms controlling sorting and transport of cargo. Vacuolar transport from the TGN/EE to multivesicular bodies/late endosomes (MVBs/LEs) is assumed to occur via clathrin-coated vesicles, although direct proof for their participation is missing. Here, we present evidence that post-TGN transport toward lytic vacuoles occurs independently of clathrin and that MVBs/LEs are derived from the TGN/EE through maturation. We show that the V-ATPase inhibitor concanamycin A significantly reduces the number of MVBs and causes TGN and MVB markers to colocalize in Arabidopsis thaliana roots. Ultrastructural analysis reveals the formation of MVBs from the TGN/EE and their fusion with the vacuole. The localization of the ESCRT components VPS28, VPS22, and VPS2 at the TGN/EE and MVBs/LEs indicates that the formation of intraluminal vesicles starts already at the TGN/EE. Accordingly, a dominant-negative mutant of VPS2 causes TGN and MVB markers to colocalize and blocks vacuolar transport. RNA interference-mediated knockdown of the annexin ANNAT3 also yields the same phenotype. Together, these data indicate that MVBs originate from the TGN/EE in a process that requires the action of ESCRT for the formation of intraluminal vesicles and annexins for the final step of releasing MVBs as a transport carrier to the vacuole.
|Item Type:||Journal Article|
|Subjects:||Q Science > QK Botany|
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Faculty of Science > Life Sciences (2010- )
|Library of Congress Subject Headings (LCSH):||Arabidopsis, Golgi apparatus, Endosomes|
|Journal or Publication Title:||Plant Cell|
|Publisher:||American Society of Plant Biologists|
|Page Range:||pp. 3463-3481|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Deutsche Forschungsgemeinschaft (DFG)|
|Grant number:||PI 769/1-1 (DFG), RO 440/14-1 (DFG)|
Actions (login required)