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Multivesicular bodies mature from the trans-golgi network/early endosome in Arabidopsis
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Scheuring, David, Viotti, Corrado, Kruger, Falco, Kunzl, Fabian, Sturm, Silke, Bubeck, Julia, Hillmer, Stefan, 1959-, Frigerio, Lorenzo, Robinson, David G., Pimpl, Peter and Schumacher, Karin. (2011) Multivesicular bodies mature from the trans-golgi network/early endosome in Arabidopsis. Plant Cell, Vol.23 (No.9). pp. 3463-3481. ISSN 1040-4651
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Official URL: http://dx.doi.org/10.1105/tpc.111.086918
Abstract
The plant trans-Golgi network/early endosome (TGN/EE) is a major hub for secretory and endocytic trafficking with complex molecular mechanisms controlling sorting and transport of cargo. Vacuolar transport from the TGN/EE to multivesicular bodies/late endosomes (MVBs/LEs) is assumed to occur via clathrin-coated vesicles, although direct proof for their participation is missing. Here, we present evidence that post-TGN transport toward lytic vacuoles occurs independently of clathrin and that MVBs/LEs are derived from the TGN/EE through maturation. We show that the V-ATPase inhibitor concanamycin A significantly reduces the number of MVBs and causes TGN and MVB markers to colocalize in Arabidopsis thaliana roots. Ultrastructural analysis reveals the formation of MVBs from the TGN/EE and their fusion with the vacuole. The localization of the ESCRT components VPS28, VPS22, and VPS2 at the TGN/EE and MVBs/LEs indicates that the formation of intraluminal vesicles starts already at the TGN/EE. Accordingly, a dominant-negative mutant of VPS2 causes TGN and MVB markers to colocalize and blocks vacuolar transport. RNA interference-mediated knockdown of the annexin ANNAT3 also yields the same phenotype. Together, these data indicate that MVBs originate from the TGN/EE in a process that requires the action of ESCRT for the formation of intraluminal vesicles and annexins for the final step of releasing MVBs as a transport carrier to the vacuole.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QK Botany |
| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Science > Life Sciences (2010- ) |
| Library of Congress Subject Headings (LCSH): | Arabidopsis, Golgi apparatus, Endosomes |
| Journal or Publication Title: | Plant Cell |
| Publisher: | American Society of Plant Biologists |
| ISSN: | 1040-4651 |
| Date: | September 2011 |
| Volume: | Vol.23 |
| Number: | No.9 |
| Page Range: | pp. 3463-3481 |
| Identification Number: | 10.1105/tpc.111.086918 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Funder: | Deutsche Forschungsgemeinschaft (DFG) |
| Grant number: | PI 769/1-1 (DFG), RO 440/14-1 (DFG) |
| URI: | http://wrap.warwick.ac.uk/id/eprint/39864 |
Data sourced from Thomson Reuters' Web of Knowledge
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