Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Identification of active bacteria involved in decomposition of complex maize and soybean residues in a tropical Vertisol using 15N-DNA stable isotope probing

Tools
- Tools
+ Tools

España, Mingrelia, Rasche, Frank, Kandeler, Ellen, Brune, Thomas, Rodriguez, Belkis, Bending, G. D. and Cadisch, G. (Georg) (2011) Identification of active bacteria involved in decomposition of complex maize and soybean residues in a tropical Vertisol using 15N-DNA stable isotope probing. Pedobiologia, Vol.54 (No.3). pp. 187-193. doi:10.1016/j.pedobi.2011.03.001

Research output not available from this repository, contact author.
Official URL: http://dx.doi.org/10.1016/j.pedobi.2011.03.001

Request Changes to record.

Abstract

As limited information is available about the relationship between microbial processes and community structure in tropical soils, we used (15)N-DNA stable isotope probing ((15)N-DNA-SIP) to identify bacteria actively involved in decomposition of plant residues of different biochemical quality. (15)N-labeled (90 atom%) and unlabeled (control) maize (C-to-N ratio: 32; cellulose content: 24.9%) and soybean (15; 15.5%) leaf residues were incubated in a tropical Vertisol for 15 days. Soil DNA was isolated, subjected to (15)N-DNA-SIP and buoyant density-resolved DNA fractions were analyzed by 16S rRNA gene-based denaturing gradient gel electrophoresis (DGGE) analysis and sequencing of selected DGGE bands. Residue addition induced new bands and changed relative intensity of already existing bands in (15)N-enriched SIP fractions. Phylogenetic analysis of selected, cloned DGGE bands from 'heaviest' (15)N-enriched fractions (57.8 atom% (maize), 87.1 atom% (soybean)) revealed that soils treated with maize residues were dominated by Pseudonocardia sp., while Arthrobacter sp. and Streptomyces sp. were found in the soybean residue treated soils. Sequences related to Bacillus sp. and Saccharopolyspora sp. were found in both organic residue treatments. Our study gave clear evidence that (15)N-DNA-SIP combined with 16S rRNA gene-based community fingerprinting of density-resolved fractions and an unlabeled control was suited for detecting active bacteria involved in decomposition of complex maize and soybean residues. In conclusion, we could show that residue quality, inducing contrasting N assimilation by decomposing bacteria, was a substantial determinant of certain decomposing community members assayed in this study.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology
S Agriculture > S Agriculture (General)
Divisions: Faculty of Science > Physics
Library of Congress Subject Headings (LCSH): Biodegradation, Bacteria, Vertisols, Molecular probes, Stable isotopes in ecological research, Stable isotopes in soil fertility research
Journal or Publication Title: Pedobiologia
Publisher: Urban und Fischer Verlag
ISSN: 0031-4056
Official Date: 2011
Dates:
DateEvent
2011Published
Volume: Vol.54
Number: No.3
Page Range: pp. 187-193
DOI: 10.1016/j.pedobi.2011.03.001
Status: Peer Reviewed
Publication Status: Published
Funder: Fondo Nacional de Ciencia, Tecnología e Innovación (Venezuela) (FONACIT), Instituto Nacional de Investigaciones Agrícolas (Venezuela) (INIA)

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us