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Mouse embryo co-culture with autologous cumulus cells and fetal development post-embryo transfer
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Omar Farouk, Farha Naomi, Stott, D. (David) and Vlad, Marcela (2011) Mouse embryo co-culture with autologous cumulus cells and fetal development post-embryo transfer. Animal Science Journal, Vol.82 (No.3). pp. 420-427. doi:10.1111/j.1740-0929.2010.00869.x ISSN 13443941.
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Official URL: http://dx.doi.org/10.1111/j.1740-0929.2010.00869.x
Abstract
This study was conducted to examine the potential for implantation and sustainable fetal development of mouse embryos cultured from the pronuclear to blastocyst stage. Pronuclear embryos from ICR mice (Harlan Sprague-Dawley) were cultured in Sydney IVF sequential media (Cook) to the blastocyst stage in medium only or co-cultured with autologous cumulus cells. We also experimented with co-culture in 100 mu L drops. Drop co-culture produced blastocyst formation rates with a mean of 47.0%, which was significantly higher (P < 0.05) compared to embryos cultured in identical culture conditions except without cumulus cells at 27.3%. Blastocysts obtained in vitro in Cook medium only and co-cultured in Cook medium with cumulus cells were transferred to pseudopregnant females of ICR strain. The day of blastocyst transfer into surrogate females was designated as post-transfer of blastocyst day 1 (PT 1). The implantation and fetal development was compared to embryo transfer of in vivo derived blastocysts, which served as controls. There were no statistical differences for implantation and fetal development rates for blastocysts cultured in vitro in either Cook medium only or co-culture in Cook medium with cumulus cells compared to in vivo-derived blastocysts. The advantage of the co-culture system is in generating more blastocysts available for transfer.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QL Zoology | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
| Library of Congress Subject Headings (LCSH): | Blastocyst, Embryo transplantation, Fetus -- Development | ||||
| Journal or Publication Title: | Animal Science Journal | ||||
| Publisher: | Wiley-Blackwell Publishing Asia | ||||
| ISSN: | 13443941 | ||||
| Official Date: | 2011 | ||||
| Dates: |
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| Volume: | Vol.82 | ||||
| Number: | No.3 | ||||
| Page Range: | pp. 420-427 | ||||
| DOI: | 10.1111/j.1740-0929.2010.00869.x | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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