Omar Farouk, Farha Naomi, Stott, D. (David) and Vlad, Marcela. (2011) Mouse embryo co-culture with autologous cumulus cells and fetal development post-embryo transfer. Animal Science Journal, Vol.82 (No.3). pp. 420-427. ISSN 13443941

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Abstract

This study was conducted to examine the potential for implantation and sustainable fetal development of mouse embryos cultured from the pronuclear to blastocyst stage. Pronuclear embryos from ICR mice (Harlan Sprague-Dawley) were cultured in Sydney IVF sequential media (Cook) to the blastocyst stage in medium only or co-cultured with autologous cumulus cells. We also experimented with co-culture in 100 mu L drops. Drop co-culture produced blastocyst formation rates with a mean of 47.0%, which was significantly higher (P < 0.05) compared to embryos cultured in identical culture conditions except without cumulus cells at 27.3%. Blastocysts obtained in vitro in Cook medium only and co-cultured in Cook medium with cumulus cells were transferred to pseudopregnant females of ICR strain. The day of blastocyst transfer into surrogate females was designated as post-transfer of blastocyst day 1 (PT 1). The implantation and fetal development was compared to embryo transfer of in vivo derived blastocysts, which served as controls. There were no statistical differences for implantation and fetal development rates for blastocysts cultured in vitro in either Cook medium only or co-culture in Cook medium with cumulus cells compared to in vivo-derived blastocysts. The advantage of the co-culture system is in generating more blastocysts available for transfer.

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Item Type:	Journal Article
Subjects:	Q Science > QL Zoology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	Blastocyst, Embryo transplantation, Fetus -- Development
Journal or Publication Title:	Animal Science Journal
Publisher:	Wiley-Blackwell Publishing Asia
ISSN:	13443941
Date:	2011
Volume:	Vol.82
Number:	No.3
Page Range:	pp. 420-427
Identification Number:	10.1111/j.1740-0929.2010.00869.x
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
URI:	http://wrap.warwick.ac.uk/id/eprint/39988

Data sourced from Thomson Reuters' Web of Knowledge

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