Spindle checkpoint silencing requires association of PP1 to both Spc7 and kinesin-8 motors
Meadows, John C., Shepperd, Lindsey A., Vanoosthuyse, Vincent, Lancaster, Theresa C., Sochaj, Alicja M., Buttrick, Graham J., Hardwick, K. G. and Millar, Jonathan B. A.. (2011) Spindle checkpoint silencing requires association of PP1 to both Spc7 and kinesin-8 motors. Developmental Cell, Vol.20 (No.6). pp. 739-750. ISSN 1534-5807Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.devcel.2011.05.008
The spindle checkpoint is the prime cell-cycle control mechanism that ensures sister chromatids are bioriented before anaphase takes place. Aurora B kinase, the catalytic subunit of the chromosome passenger complex, both destabilizes kinetochore attachments that do not generate tension and simultaneously maintains the spindle checkpoint signal. However, it is unclear how the checkpoint is silenced following chromosome biorientation. We demonstrate. that association of type 1 phosphatase (PP1(Dis2)) with both the N terminus of Spc7 and the nonmotor domains of the Klp5-Klp6 (kinesin-8) complex is necessary to counteract Aurora B kinase to efficiently silence the spindle checkpoint. The role of Klp5 and Klp6 in checkpoint silencing is specific to this class of kinesin and independent of their motor activities. These data demonstrate that at least two distinct pools of PP1, one kinetochore associated and the other motor associated, are needed to silence the spindle checkpoint.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology
Q Science > QP Physiology
|Divisions:||Faculty of Medicine > Warwick Medical School > Biomedical Cell Biology
Faculty of Medicine > Warwick Medical School
|Library of Congress Subject Headings (LCSH):||Cell cycle -- Regulation, Kinesin|
|Journal or Publication Title:||Developmental Cell|
|Official Date:||14 June 2011|
|Page Range:||pp. 739-750|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Medical Research Council (Great Britain) (MRC), Wellcome Trust (London, England)|
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