Flaxman, Christine Susan (1995) A study of the regulation of undecylprodigiosin biosynthesis in Streptomyces coelicolor A3(2). PhD thesis, University of Warwick.

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Abstract

Undecylprodigiosin is one of four secondary metabolites with antibacterial
activity produced by S. coelicolor A3(2). The overall aim of this study was to
further investigate the control of biosynthesis of the secondary metabolite
undecylprodigiosin (Red) in Streptomyces coelicolor A3(2).
Proline transport mutants (Put-) were isolated, and the over-production of Red
was observed in these strains. It was hypothesised that Red biosynthesis is
essential as a shunt for excess proline in the Put- mutants. Red biosynthesis was
abolished by disrupting the redX structural gene in a Put- mutant. The Put-
RedX- mutants were viable, demonstrating that Red is not essential in Pur
mutants. Si nuclease mapping of redD and redX genes in a Put- mutant revealed
that red genes are transcribed earlier in the growth phase of Put- mutants
compared to the progenitor strain J802.
Pwb (pigmented whilst bid) mutants had been isolated due to their ability to
produce Red in a b1dA background. The regions believed to contain the
mutations of Pwb-6, Pwb-9, Pwb-16 and Pwb+ were sub-cloned and sequence
data obtained. An open reading frame was identified which is predicted to
encode a protein showing homology to the UhpA-LuxR family of regulators. The
open reading frame, contains an in-frame TTA codon. It is proposed that this
gene, named redZ, mediates the b1dA dependence of Red biosynthesis. The
Pwb-6 mutation was located to the putative -35 promoter region. The mutation
makes the promoter more similar to the enteric bacterium major sigma factor
promoter -35 consensus sequence. It is anticipated that greater transcription from
the promoter causes the Pwb phenotype. Introduction of the Pwb-9 redZ gene
into antibiotic biosynthesis mutants, absA and absB, did not result in Red
biosynthesis.

Item Type:	Thesis (PhD)
Subjects:	Q Science > QR Microbiology
Library of Congress Subject Headings (LCSH):	Streptomyces coelicolor -- Metabolism, Microbial metabolites -- Synthesis, Antibiotics -- Synthesis
Official Date:	September 1995
Dates:	Date Event September 1995 Submitted
Institution:	University of Warwick
Theses Department:	Department of Biological Sciences
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Hodgson, D. A. (David A.)
Extent:	xix, 230 p.
Language:	eng

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