Identification of the tomato ABA-deficient mutant sitiens as a member of the ABA-aldehyde oxidase gene family using genetic and genomic analysis
Harrison, Elizabeth, Burbidge, A., Okyere, J. P., Thompson, Andrew J. and Taylor, Ian B.. (2011) Identification of the tomato ABA-deficient mutant sitiens as a member of the ABA-aldehyde oxidase gene family using genetic and genomic analysis. Plant Growth Regulation, Vol.64 (No.3). pp. 301-309. ISSN 0167-6903Full text not available from this repository.
Official URL: http://dx.doi.org/10.1007/s10725-010-9550-1
The sitiens (sit) wilty mutant of tomato (Solanum lycopersicum L.) is deficient in functional enzyme activity at the final step in abscisic acid (ABA) biosynthesis. The biochemical lesion is believed to be an impaired aldehyde oxidase (AO). Molecular mapping using various interspecies crosses has previously shown sit to co-map with a cluster of unresolved RFLP markers on the short arm of chromosome 1. Here, the utilisation of bridging lines to produce interspecies mapping populations involving a self-compatible S. peruvianum accession (LA2157) allowed the fine mapping of sit within this cluster. Identification of a novel AO gene, within the region now known to contain the sit locus, was confirmed by analysis of the tomato whole genome shotgun sequence assembly. This novel AO protein shares 76-78% identity at the amino acid level with the previously characterised tomato AO proteins. The DNA sequence of this putative sit gene was characterised in wild type and in two allelic sit mutants (sit and sit (w)): changes in DNA sequence were identified in these mutant alleles that cause a truncation of exon 2 and the deletion of exon 7, respectively. These results establish the identity of the tomato sit gene and are consistent with its proposed function of encoding the ABA aldehyde oxidase apoenzyme.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history
Q Science > QK Botany
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)|
|Library of Congress Subject Headings (LCSH):||Tomatoes, Abscisic acid, Aldehydes, Tomatoes -- Genome mapping|
|Journal or Publication Title:||Plant Growth Regulation|
|Official Date:||July 2011|
|Page Range:||pp. 301-309|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Great Britain. Dept. for Environment, Food & Rural Affairs (DEFRA), University of Nottingham, Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)|
|Grant number:||PG42/646 (BBSRC), WU0106 (DEFRA)|
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