Harrison, Elizabeth, Burbidge, A., Okyere, J. P., Thompson, Andrew J. and Taylor, Ian B.. (2011) Identification of the tomato ABA-deficient mutant sitiens as a member of the ABA-aldehyde oxidase gene family using genetic and genomic analysis. Plant Growth Regulation, Vol.64 (No.3). pp. 301-309. ISSN 0167-6903

Full text not available from this repository.

Abstract

The sitiens (sit) wilty mutant of tomato (Solanum lycopersicum L.) is deficient in functional enzyme activity at the final step in abscisic acid (ABA) biosynthesis. The biochemical lesion is believed to be an impaired aldehyde oxidase (AO). Molecular mapping using various interspecies crosses has previously shown sit to co-map with a cluster of unresolved RFLP markers on the short arm of chromosome 1. Here, the utilisation of bridging lines to produce interspecies mapping populations involving a self-compatible S. peruvianum accession (LA2157) allowed the fine mapping of sit within this cluster. Identification of a novel AO gene, within the region now known to contain the sit locus, was confirmed by analysis of the tomato whole genome shotgun sequence assembly. This novel AO protein shares 76-78% identity at the amino acid level with the previously characterised tomato AO proteins. The DNA sequence of this putative sit gene was characterised in wild type and in two allelic sit mutants (sit and sit (w)): changes in DNA sequence were identified in these mutant alleles that cause a truncation of exon 2 and the deletion of exon 7, respectively. These results establish the identity of the tomato sit gene and are consistent with its proposed function of encoding the ABA aldehyde oxidase apoenzyme.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history Q Science > QK Botany
Divisions:	Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Tomatoes, Abscisic acid, Aldehydes, Tomatoes -- Genome mapping
Journal or Publication Title:	Plant Growth Regulation
Publisher:	Springer Netherlands
ISSN:	0167-6903
Official Date:	July 2011
Volume:	Vol.64
Number:	No.3
Page Range:	pp. 301-309
Identification Number:	10.1007/s10725-010-9550-1
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Great Britain. Dept. for Environment, Food & Rural Affairs (DEFRA), University of Nottingham, Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)
Grant number:	PG42/646 (BBSRC), WU0106 (DEFRA)
URI:	http://wrap.warwick.ac.uk/id/eprint/40012

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Actions (login required)

View Item