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Response to mercury (II) ions in Methylococcus capsulatus (Bath)
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Boden, Rich and Murrell, J. C. (J. Colin) (2011) Response to mercury (II) ions in Methylococcus capsulatus (Bath). FEMS Microbiology Letters, Vol.324 (No.2). pp. 106-110. doi:10.1111/j.1574-6968.2011.02395.x
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Official URL: http://dx.doi.org/10.1111/j.1574-6968.2011.02395.x
Abstract
The mercury (II) ion is toxic and is usually detoxified in Bacteria by reduction to elemental mercury, which is less toxic. This is catalysed by an NAD(P)H-dependent mercuric reductase (EC 1.16.1.1). Here, we present strong evidence that Methylococcus capsulatus (Bath) - a methanotrophic member of the Gammaproteobacteria - uses this enzyme to detoxify mercury. In radiorespirometry studies, it was found that cells exposed to mercury dissimilated 100% of [(14)C]methane provided to generate reducing equivalents to fuel mercury (II) reduction, rather than the mix of assimilation and dissimilation found in control incubations. The detoxification system is constitutively expressed with a specific activity of 352 (+/- 18) nmol NADH oxidized min(-1) (mg protein)(-1). Putative mercuric reductase genes were predicted in the M. capsulatus (Bath) genome and found in mRNA microarray studies. The MerA-derived polypeptide showed high identity (> 80%) with MerA sequences from the Betaproteobacteria.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QP Physiology Q Science > QR Microbiology |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||
| Library of Congress Subject Headings (LCSH): | Heavy metals -- Physiological effect, Flavoproteins, Methanotrophs, Oxidoreductases, Metal ions -- Metabolism | ||||
| Journal or Publication Title: | FEMS Microbiology Letters | ||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
| ISSN: | 0378-1097 | ||||
| Official Date: | November 2011 | ||||
| Dates: |
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| Volume: | Vol.324 | ||||
| Number: | No.2 | ||||
| Page Range: | pp. 106-110 | ||||
| DOI: | 10.1111/j.1574-6968.2011.02395.x | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
Data sourced from Thomson Reuters' Web of Knowledge
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