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Insights into the stereospecificity of ketoreduction in a modular polyketide synthase
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Kwan, David H., Tosin, Manuela, Schläger, Nadin, Schulz, F. (Frank) and Leadlay, P. F. (2011) Insights into the stereospecificity of ketoreduction in a modular polyketide synthase. Organic & Biomolecular Chemistry, Vol.9 (No.7). pp. 2053-2056. doi:10.1039/c1ob00022e ISSN 1477-0520.
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Official URL: http://dx.doi.org/10.1039/C1OB00022E
Abstract
Ketoreductase enzymes are responsible for the generation of hydroxyl stereocentres during the biosynthesis of complex polyketide natural products. Previous studies of isolated polyketide ketoreductases have shown that the stereospecificity of ketoreduction can be switched by mutagenesis of selected active site amino acids. We show here that in the context of the intact polyketide synthase multienzyme the same changes do not alter the stereochemical outcome in the same way. These findings point towards additional factors that govern ketoreductase stereospecificity on intact multienzymes in vivo.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Library of Congress Subject Headings (LCSH): | Stereochemistry, Polyketides, Enzymes, Biosynthesis | ||||
Journal or Publication Title: | Organic & Biomolecular Chemistry | ||||
Publisher: | Royal Society of Chemistry | ||||
ISSN: | 1477-0520 | ||||
Official Date: | 2011 | ||||
Dates: |
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Volume: | Vol.9 | ||||
Number: | No.7 | ||||
Page Range: | pp. 2053-2056 | ||||
DOI: | 10.1039/c1ob00022e | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), University of Cambridge Herchel Smith Fund, University of Cambridge. Cambridge Commonwealth Trusts, Overseas Research Students Award Scheme (ORSAS), Natural Sciences and Engineering Research Council Canada (NSERC), Sixth Framework Programme (European Commission) (FP6) | ||||
Grant number: | 8/B18119 (BBSRC) |
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