Sun, Yuhui, Hahn, F., Demydchuk, Yuliya, Chettle, James, Tosin, Manuela, Osada, H. (Hiroyuki), 1954- and Leadlay, Peter F.. (2010) In vitro reconstruction of tetronate RK-682 biosynthesis. Nature Chemical Biology, Vol.6 (No.2). pp. 99-101. ISSN 1552-4450

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Abstract

The protein phosphatase inhibitor RK-682 is one of a number of potentially valuable tetronate polyketide natural products. Understanding how the tetronate ring is formed has been frustrated by the inaccessibility of the putative substrates. We report the heterologous expression of rk genes in Saccharopolyspora erythraea and reconstitution of the RK-682 pathway using recombinant enzymes, and we show that RkD is the enzyme required for RK-682 formation from acyl carrier protein–bound substrates.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QP Physiology
Divisions:	Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH):	Polyketides, Phosphoprotein phosphatases -- Inhibitors, Enzymes, Polyketides -- Synthesis
Journal or Publication Title:	Nature Chemical Biology
Publisher:	Nature Publishing Group
ISSN:	1552-4450
Official Date:	2010
Volume:	Vol.6
Number:	No.2
Page Range:	pp. 99-101
Identification Number:	10.1038/nchembio.285
Status:	Peer Reviewed
Publication Status:	Published
Funder:	Deutsche Forschungsgemeinschaft (DFG), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), University of Cambridge Herchel Smith Fund, Seventh Framework Programme (European Commission) (FP7)
Grant number:	BB/D018943/1 (BBSRC)
URI:	http://wrap.warwick.ac.uk/id/eprint/40358

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