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In vitro reconstruction of tetronate RK-682 biosynthesis
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Sun, Yuhui, Hahn, F., Demydchuk, Yuliya, Chettle, James, Tosin, Manuela, Osada, H. (Hiroyuki), 1954- and Leadlay, Peter F.. (2010) In vitro reconstruction of tetronate RK-682 biosynthesis. Nature Chemical Biology, Vol.6 (No.2). pp. 99-101. ISSN 1552-4450
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Official URL: http://dx.doi.org/10.1038/nchembio.285
Abstract
The protein phosphatase inhibitor RK-682 is one of a number of potentially valuable tetronate polyketide natural products. Understanding how the tetronate ring is formed has been frustrated by the inaccessibility of the putative substrates. We report the heterologous expression of rk genes in Saccharopolyspora erythraea and reconstitution of the RK-682 pathway using recombinant enzymes, and we show that RkD is the enzyme required for RK-682 formation from acyl carrier protein–bound substrates.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
| Divisions: | Faculty of Science > Chemistry |
| Library of Congress Subject Headings (LCSH): | Polyketides, Phosphoprotein phosphatases -- Inhibitors, Enzymes, Polyketides -- Synthesis |
| Journal or Publication Title: | Nature Chemical Biology |
| Publisher: | Nature Publishing Group |
| ISSN: | 1552-4450 |
| Date: | 2010 |
| Volume: | Vol.6 |
| Number: | No.2 |
| Page Range: | pp. 99-101 |
| Identification Number: | 10.1038/nchembio.285 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Funder: | Deutsche Forschungsgemeinschaft (DFG), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), University of Cambridge Herchel Smith Fund, Seventh Framework Programme (European Commission) (FP7) |
| Grant number: | BB/D018943/1 (BBSRC) |
| URI: | http://wrap.warwick.ac.uk/id/eprint/40358 |
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