The Library
The role of glutathione S-transferase GliG in gliotoxin biosynthesis in aspergillus fumigatus
Tools
Tools
Tools
Davis, Carol, Carberry, Stephen, Schrettl, Markus, Singh, Ishwar, Stephens, John C., Barry, Sarah M., Kavanagh, Kevin, Challis, Gregory L., Brougham, Dermot and Doyle, S. (Sean) (2011) The role of glutathione S-transferase GliG in gliotoxin biosynthesis in aspergillus fumigatus. Chemistry & Biology, Vol.18 (No.4). pp. 542-552. doi:10.1016/j.chembiol.2010.12.022
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1016/j.chembiol.2010.12.022
Abstract
Gliotoxin, a redox-active metabolite, is produced by the opportunistic fungal pathogen Aspergillus fumigatus, and its biosynthesis is directed by the gli gene cluster. Knowledge of the biosynthetic pathway to gliotoxin, which contains a disulfide bridge of unknown origin, is limited, although L-Phe and L-Ser are known biosynthetic precursors. Deletion of gliG from the gli cluster, herein functionally confirmed as a glutathione S-transferase, results in abrogation of gliotoxin biosynthesis and accumulation of 6-benzyl-6-hydroxy-1-methoxy-3-methylenepiperazine-2,5-dione. This putative shunt metabolite from the gliotoxin biosynthetic pathway contains an intriguing hydroxyl group at C-6, consistent with a gliotoxin biosynthetic pathway involving thiolation via addition of the glutathione thiol group to a reactive acyl imine intermediate. Complementation of gliG restored gliotoxin production and, unlike gliT, gliG was found not to be involved in fungal self-protection against gliotoxin.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QD Chemistry Q Science > QK Botany Q Science > QP Physiology |
||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
| Library of Congress Subject Headings (LCSH): | Aspergillus fumigatus, Mycotoxins -- Synthesis, Glutathione transferase | ||||
| Journal or Publication Title: | Chemistry & Biology | ||||
| Publisher: | Cell Press | ||||
| ISSN: | 1074-5521 | ||||
| Official Date: | 22 April 2011 | ||||
| Dates: |
|
||||
| Volume: | Vol.18 | ||||
| Number: | No.4 | ||||
| Page Range: | pp. 542-552 | ||||
| DOI: | 10.1016/j.chembiol.2010.12.022 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Science Foundation Ireland (SFI), Programme for Research in Third-Level Institutions (PRTLI), European Commission (EC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||
| Grant number: | RFP/GEN/F571 (SFI), BB/H006281/1 (BBSRC) |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
