Dawkar, Vishal V., Chikate, Yojana R., Gupta, Vidya S., Slade, Susan E. and Giri, Ashok P.. (2011) Assimilatory potential of Helicoverpa armigera reared on host (Chickpea) and nonhost (Cassia tora) diets. Journal of Proteome Research, Vol.10 (No.11). pp. 5128-5138. ISSN 1535-3893

Full text not available from this repository.

Abstract

Adaptation to plant allelochemicals is a crucial aspect of herbivore chemical ecology. To understand an insect ecology, we studied an effect of nonhost Cassia tora seed-based diet (Ct) on growth, development, and molecular responses in Helicoverpa armigera. We employed a comparative approach to investigate the proteomic differences in gut, hemolymph, and frass of H. armigera reared on a normal (chickpea seed-based, Cp) and Ct diet. In this study, a total of 46 proteins were identified by nano-LC-MS(E). Among them, 17 proteins were up-regulated and 29 proteins were down-regulated when larvae were exposed to the Ct diet. Database searches combined with GO analysis revealed that gut proteases engrossed in digestion, proteins crucial for immunity, adaptive responses to stress, and detoxification were down-regulated in the Ct fed larvae. Proteins identified in H. armigera hemolymph were found to be involved in defense mechanisms. Moreover, proteins found in frass of the Ct fed larvae were observed to participate in energy metabolism. Biochemical and quantitative real-time PCR analysis of selected candidate proteins showed differential gene expression patterns and corroborated with the proteomic data. Our results suggest that the Ct diet could alter expression of proteins related to digestion, absorption of nutrients, adaptation, defense mechanisms, and energy metabolism in H. armigera.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Helicoverpa armigera
Journal or Publication Title:	Journal of Proteome Research
Publisher:	American Chemical Society
ISSN:	1535-3893
Date:	November 2011
Volume:	Vol.10
Number:	No.11
Page Range:	pp. 5128-5138
Identification Number:	10.1021/pr200591m
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Council of Scientific & Industrial Research (India) (CSIR)
Grant number:	NWP 0003 (CSIR)
URI:	http://wrap.warwick.ac.uk/id/eprint/40424

Data sourced from Thomson Reuters' Web of Knowledge

Request changes to a record

Actions (login required)

View Item