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Measurement of site-specific 13C spin−lattice relaxation in a crystalline protein
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Lewandowski, Józef R., Sein, Julien, Sass, Hans Jürgen, Grzesiek, Stephan, Blackledge, Martin and Emsley, Lyndon (2010) Measurement of site-specific 13C spin−lattice relaxation in a crystalline protein. Journal of the American Chemical Society, Vol.132 (No.24). pp. 8252-8254. doi:10.1021/ja102744b ISSN 0002-7863.
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Official URL: http://dx.doi.org/10.1021/ja102744b
Abstract
We demonstrate that it is possible to record site-specific spin-lattice relaxation rates for the majority of (13)C sites in uniformly (13)C and (15)N labeled solid proteins as a result of the slowing down of proton-driven spin diffusion at sample spinning frequencies >= 60 kHz, thus providing a series of new experimental probes for characterizing molecular dynamics in solid proteins.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QC Physics Q Science > QD Chemistry |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Library of Congress Subject Headings (LCSH): | Spin-lattice relaxation, Proteins -- Structure, Proteins -- Analysis, Nuclear magnetic resonance spectroscopy, Molecular dynamics | ||||
Journal or Publication Title: | Journal of the American Chemical Society | ||||
Publisher: | American Chemical Society | ||||
ISSN: | 0002-7863 | ||||
Official Date: | 23 June 2010 | ||||
Dates: |
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Volume: | Vol.132 | ||||
Number: | No.24 | ||||
Page Range: | pp. 8252-8254 | ||||
DOI: | 10.1021/ja102744b | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | France. Agence nationale de la recherche (ANR), Sixth Framework Programme (European Commission) (FP6), European Union (EU) | ||||
Grant number: | RII3-026145 (FP6), PIRG03-GA-2008-231026 (EU) |
Data sourced from Thomson Reuters' Web of Knowledge
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