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15N−15N proton assisted recoupling in magic angle spinning NMR
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Lewandowski, Józef R., Paëpe, Gaël De, Eddy, Matthew T. and Griffin, Robert G. (2009) 15N−15N proton assisted recoupling in magic angle spinning NMR. Journal of the American Chemical Society, Vol.131 (No.16). pp. 5769-5776. doi:10.1021/ja806578y ISSN 0002-7863.
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Official URL: http://dx.doi.org/10.1021/ja806578y
Abstract
We describe a new magic angle spinning (MAS) NMR experiment for obtaining (15)N-(15)N correlation spectra. The approach yields direct information about the secondary and tertiary structure of proteins, including identification of alpha-helical stretches and interstrand connectivity in antiparallel beta-sheets, which are of major interest for structural studies of membrane proteins and amyloid fibrils. The method, (15)N-(15)N proton assisted recoupling (PAR), relies on a second-order mechanism, third spin assisted recoupling (TSAR), used previously in the context of (15)N-(13)C and (13)C-(13)C polarization transfer schemes. In comparison to (15)N-(15)N proton-driven spin diffusion experiments, the PAR technique accelerates polarization transfer between (15)N's by a factor of similar to 10(2)-10(3) and is furthermore applicable over the entire range of currently available MAS frequencies (10-70 kHz).
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QC Physics Q Science > QD Chemistry |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
| Library of Congress Subject Headings (LCSH): | Nuclear magnetic resonance spectroscopy, Proteins -- Analysis, Protons, Nuclear spin | ||||
| Journal or Publication Title: | Journal of the American Chemical Society | ||||
| Publisher: | American Chemical Society | ||||
| ISSN: | 0002-7863 | ||||
| Official Date: | 29 April 2009 | ||||
| Dates: |
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| Volume: | Vol.131 | ||||
| Number: | No.16 | ||||
| Page Range: | pp. 5769-5776 | ||||
| DOI: | 10.1021/ja806578y | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | National Institutes of Health (U.S.) (NIH) | ||||
| Grant number: | EB001960 (NIH), EB-003151 (NIH), EB-002026 (NIH) |
Data sourced from Thomson Reuters' Web of Knowledge
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