The Library
Proton assisted recoupling at high spinning frequencies
Tools
Tools
Tools
Lewandowski, Józef R., Paëpe, Gaël De, Eddy, Matthew T., Struppe, Jochem, Maas, Werner and Griffin, Robert G. (2009) Proton assisted recoupling at high spinning frequencies. Journal of Physical Chemistry B, Vol.113 (No.27). pp. 9062-9069. doi:10.1021/jp810280t ISSN 1520-6106.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1021/jp810280t
Abstract
We demonstrate the successful application of (13)C-(13)C proton assisted recoupling (PAR) on [U-(13)C,(15)N] N-f-MLF-OH and [U-(13)C,(15)N] protein GBI at high magic angle spinning (MAS) frequencies (omega(r)/2 pi = 65 kHz). Specifically, by combining PAR mixing with low power heteronuclear decoupling (omega(1H)/2(pi) similar to 16 kHz) and high spinning frequencies, we obtain high resolution 2D spectra displaying long-range (13)C-(13)C contacts from which distance estimates call be extracted. These experiments therefore demonstrate the possibility of performing high resolution Structural studies in the limit of high spinning frequency and low power (1)H decoupling, a regime which optimizes the resolution of protein samples and preserves their integrity.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QC Physics Q Science > QD Chemistry |
||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
| Library of Congress Subject Headings (LCSH): | Nuclear spin, Nuclear magnetic resonance spectroscopy, Protons, Proteins -- Analysis | ||||
| Journal or Publication Title: | Journal of Physical Chemistry B | ||||
| Publisher: | American Chemical Society | ||||
| ISSN: | 1520-6106 | ||||
| Official Date: | 9 July 2009 | ||||
| Dates: |
|
||||
| Volume: | Vol.113 | ||||
| Number: | No.27 | ||||
| Page Range: | pp. 9062-9069 | ||||
| DOI: | 10.1021/jp810280t | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | National Institutes of Health (U.S.) (NIH) | ||||
| Grant number: | EB-003151 (NIH), EB-001960 (NIH), EB-002026 (NIH) |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
