
The Library
Probing the gas-phase folding kinetics of peptide ions by IR activated DR-ECD
Tools
Lin, Cheng, Cournoyer, Jason J. and O'Connor, Peter B. (2008) Probing the gas-phase folding kinetics of peptide ions by IR activated DR-ECD. Journal of The American Society for Mass Spectrometry, Vol.19 (No.6). pp. 780-789. doi:10.1016/j.jasms.2008.01.001 ISSN 1044-0305.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1016/j.jasms.2008.01.001
Abstract
The effect of infrared (IR) irradiation on the electron capture dissociation (ECD) fragmentation pattern of peptide ions was investigated. IR heating increases the internal energy of the precursor ion, which often amplifies secondary fragmentation, resulting in the formation of w-type ions as well as other secondary fragments. Improved sequence coverage was observed with IR irradiation before ECD, likely due to the increased conformational heterogeneity upon IR heating, rather than faster breakdown of the initially formed product ion complex, as IR heating after ECD did not have similar effect. Although the ECD fragment ion yield of peptide ions does not typically increase with IR heating, in double resonance (DR) ECD experiments, fragment ion yield may be reduced by fast resonant ejection of the charge reduced molecular species, and becomes dependent on the folding state of the precursor ion. In this work, the fragment ion yield was monitored as a function of the delay between IR irradiation and the DR-ECD event to study the gas-phase folding kinetics of the peptide ions. Furthermore, the degree of intracomplex hydrogen transfer of the ECD fragment ion pair was used to probe the folding state of the precursor ion. Both methods gave similar refolding time constants of ∼1.5 s−1, revealing that gaseous peptide ions often refold in less than a second, much faster than their protein counterparts. It was also found from the IR-DR-ECD study that the intramolecular H· transfer rate can be an order of magnitude higher than that of the separation of the long-lived c/z product ion complexes, explaining the common observation of c· and z type ions in ECD experiments.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Library of Congress Subject Headings (LCSH): | Tandem mass spectrometry, Protein folding -- Research, Electrospray ionization mass spectrometry, Ions, Post-translational modification | ||||
Journal or Publication Title: | Journal of The American Society for Mass Spectrometry | ||||
Publisher: | Springer New York LLC | ||||
ISSN: | 1044-0305 | ||||
Official Date: | June 2008 | ||||
Dates: |
|
||||
Volume: | Vol.19 | ||||
Number: | No.6 | ||||
Number of Pages: | 10 | ||||
Page Range: | pp. 780-789 | ||||
DOI: | 10.1016/j.jasms.2008.01.001 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Funder: | National Institutes of Health (U.S.) (NIH), National Institute of General Medical Sciences (U.S.) (NIGMS), National Heart, Lung, and Blood Institute (NHLBI), National Center for Research Resources (U.S.) (NCRR), MDS Sciex, Petroleum Research Fund | ||||
Grant number: | P41RR10888 (NIH/NCRR), N01HV28178 (NIH/NHLBI), R01GM078293 (NIH/NIGMS) |
Data sourced from Thomson Reuters' Web of Knowledge
Request changes or add full text files to a record
Repository staff actions (login required)
![]() |
View Item |