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Genome organization and transcription response to harvest of two Metallothionein-like genes in agaricus bisporus fruiting bodies

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Eastwood, Daniel C., Bains, N. K., Henderson, Janey and Burton, Kerry S. (2011) Genome organization and transcription response to harvest of two Metallothionein-like genes in agaricus bisporus fruiting bodies. Journal of Microbiology and Biotechnology, Volume 21 (Number 5). pp. 455-463. doi:10.4014/jmb.1007.07031

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Official URL: http://dx.doi.org/10.4014/jmb.1007.07031

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Abstract

Metallothioneins are a class of small cysteine-rich proteins that have been associated with increased tolerance to metal and oxidative stresses in animals, plants, and fungi. We investigated a metallothionein-like (mt-like) gene shown previously to be upregulated in fruiting bodies of the fungus Agaricus bisporus in response to post-harvest storage. Analysis of an A. bisporus genomic DNA cosmid library identified two similar nit-like genes (men and met2) arranged as a bidirectional gene pair transcribed from the same promoter region. The promoter contained regulatory elements including 9 metal responsive elements and a CAAT box region 220 bp downstream of met1 that showed striking similarity to a feature in Coprinopsis cinerea mt-like gene promoters. Transcriptional analysis showed that both met genes are significantly and rapidly (within 3 hours) upregulated during post-harvest storage and expression is significantly greater in stipe and cap tissues compared with the gills. However, a strong directionality of the promoter was demonstrated, as transcript levels of met1 were at least two orders of magnitude greater than those of met2 in all samples tested

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH426 Genetics
Q Science > QK Botany
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Plant genomes, Genetic transcription, Metallothionein, Cultivated mushroom -- Genetics
Journal or Publication Title: Journal of Microbiology and Biotechnology
Publisher: Han'gug Mi'saengmul Saengmyeong Gong Haghoe
ISSN: 1017-7825
Official Date: 2011
Dates:
DateEvent
2011Published
Volume: Volume 21
Number: Number 5
Page Range: pp. 455-463
DOI: 10.4014/jmb.1007.07031
Status: Peer Reviewed
Publication Status: Published
Funder: Great Britain. Department for Environment, Food & Rural Affairs (DEFRA)
Grant number: HH2116SMU (DEFRA)

Data sourced from Thomson Reuters' Web of Knowledge

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