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Genome organization and transcription response to harvest of two Metallothionein-like genes in agaricus bisporus fruiting bodies
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Eastwood, Daniel C., Bains, N. K., Henderson, Janey and Burton, Kerry S. (2011) Genome organization and transcription response to harvest of two Metallothionein-like genes in agaricus bisporus fruiting bodies. Journal of Microbiology and Biotechnology, Volume 21 (Number 5). pp. 455-463. doi:10.4014/jmb.1007.07031
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Official URL: http://dx.doi.org/10.4014/jmb.1007.07031
Abstract
Metallothioneins are a class of small cysteine-rich proteins that have been associated with increased tolerance to metal and oxidative stresses in animals, plants, and fungi. We investigated a metallothionein-like (mt-like) gene shown previously to be upregulated in fruiting bodies of the fungus Agaricus bisporus in response to post-harvest storage. Analysis of an A. bisporus genomic DNA cosmid library identified two similar nit-like genes (men and met2) arranged as a bidirectional gene pair transcribed from the same promoter region. The promoter contained regulatory elements including 9 metal responsive elements and a CAAT box region 220 bp downstream of met1 that showed striking similarity to a feature in Coprinopsis cinerea mt-like gene promoters. Transcriptional analysis showed that both met genes are significantly and rapidly (within 3 hours) upregulated during post-harvest storage and expression is significantly greater in stipe and cap tissues compared with the gills. However, a strong directionality of the promoter was demonstrated, as transcript levels of met1 were at least two orders of magnitude greater than those of met2 in all samples tested
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QK Botany |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Library of Congress Subject Headings (LCSH): | Plant genomes, Genetic transcription, Metallothionein, Cultivated mushroom -- Genetics | ||||
| Journal or Publication Title: | Journal of Microbiology and Biotechnology | ||||
| Publisher: | Han'gug Mi'saengmul Saengmyeong Gong Haghoe | ||||
| ISSN: | 1017-7825 | ||||
| Official Date: | 2011 | ||||
| Dates: |
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| Volume: | Volume 21 | ||||
| Number: | Number 5 | ||||
| Page Range: | pp. 455-463 | ||||
| DOI: | 10.4014/jmb.1007.07031 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Funder: | Great Britain. Department for Environment, Food & Rural Affairs (DEFRA) | ||||
| Grant number: | HH2116SMU (DEFRA) |
Data sourced from Thomson Reuters' Web of Knowledge
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