Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Increased drying rate lowers the critical water content for survival in embryonic axes of English oak (Quercus robur L.) seeds

Tools
- Tools
+ Tools

Ntuli, Tobias M., Finch-Savage, William E., Berjak, Patricia and Pammenter, Norman W. (2011) Increased drying rate lowers the critical water content for survival in embryonic axes of English oak (Quercus robur L.) seeds. Journal of Integrative Plant Biology, Volume 53 (Number 4). pp. 270-280. doi:10.1111/j.1744-7909.2010.01016.x

Research output not available from this repository, contact author.
Official URL: http://dx.doi.org/10.1111/j.1744-7909.2010.01016.x

Request Changes to record.

Abstract

The potential to cryopreserve embryonic axes of desiccation-sensitive (recalcitrant) seeds is limited by damage during the desiccation necessary for low temperature survival, but the basis of this injury and how to reduce it is not well understood. The effects of drying rate on the viability, respiratory metabolism and free radical-mediated processes were therefore investigated during dehydration of Quercus robur L. embryonic axes. Viability, assessed by evidence of germination and tetrazolium staining, showed a sharp decline at 0.27 and 0.8 g/g during rapid (<12 h) or slow (3 d) dehydration, respectively. Rapid dehydration therefore lowered the critical water content for survival. At any given water content rapid dehydration was associated with higher activities of the free radical processing enzymes, superoxide dismutase, catalase and glutathione reductase and lower levels of hydroperoxide and membrane damage. Rapid dehydration was also associated with lower malate dehydrogenase activity, and a reduced decline in phosphofructokinase activity and in levels of the oxidized form of nicotinamide dinucleotide. Ageing may have contributed to increased damage during slow dehydration, since viability declined even in hydrated storage after 3 d. The results presented are consistent with rapid dehydration reducing the accumulation of damage resulting from desiccation induced aqueous-based deleterious reactions.

Item Type: Journal Article
Subjects: Q Science > QK Botany
S Agriculture > SB Plant culture
Divisions: Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH): English oak -- Seeds -- Drying, English oak -- Seeds -- Storage, English oak -- Seeds -- Viability
Journal or Publication Title: Journal of Integrative Plant Biology
Publisher: Wiley-Blackwell Publishing Ltd.
ISSN: 16729072
Official Date: April 2011
Dates:
DateEvent
April 2011Published
Volume: Volume 53
Number: Number 4
Page Range: pp. 270-280
DOI: 10.1111/j.1744-7909.2010.01016.x
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: National Research Foundation (South Africa)

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us