The Library

Acetate repression of methane oxidation by supplemental methylocella silvestris in a peat soil microcosm

Tools

Rahman, M. Tanvir, Crombie, Andrew, Moussard, Hélène, Chen, Y. (Yin) and Murrell, J. C. (J. Colin). (2011) Acetate repression of methane oxidation by supplemental methylocella silvestris in a peat soil microcosm. Applied and Environmental Microbiology, Volume 77 (Number 12). pp. 4234-4236. ISSN 0099-2240

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1128/AEM.02902-10

Abstract

Methylocella spp. are facultative methanotrophs that grow on methane and multicarbon substrates, such as acetate. Acetate represses transcription of methane monooxygenase of Methylocella silvestris in laboratory culture. DNA stable-isotope probing (DNA-SIP) using (13)C-methane and (12)C-acetate, carried out with Methylocella-spiked peat soil, showed that acetate also repressed methane oxidation by Methylocella in environmental samples.

Item Type:

Journal Article

Subjects:

Q Science > QR Microbiology

Divisions:

Faculty of Science > Life Sciences (2010- )

Library of Congress Subject Headings (LCSH):

Methanotrophs, Acetates, Methane -- Oxidation

Journal or Publication Title:

Applied and Environmental Microbiology

Publisher:

American Society for Microbiology

ISSN:

0099-2240

Official Date:

2011

Dates:

Date	Event
2011	Published

Volume:

Volume 77

Number:

Number 12

Page Range:

pp. 4234-4236

Identification Number:

10.1128/AEM.02902-10

Status:

Peer Reviewed

Publication Status:

Published

Funder:

Natural Environment Research Council (Great Britain) (NERC), University of Warwick

Grant number:

NE/E016855/1 (NERC)

References:

1. Belova, S. E., et al. 2011. Acetate utilization as a survival strategy of peatinhabiting
Methylocystis spp. Environ. Microbiol. Rep. 3:36–46.
2. Chen, Y., et al. 2008. Diversity of the active methanotrophic community in
acidic peatlands as assessed by mRNA and SIP-PLFA analyses. Environ.
Microbiol. 10:446–459.
3. Chen, Y., et al. 2008. Revealing the uncultivated majority: combining DNA
stable-isotope probing, multiple displacement amplification and metagenomic
analyses of uncultivated Methylocystis in acidic peatlands. Environ.
Microbiol. 10:2609–2622.
4. Dedysh, S. N., et al. 2000. Methylocella palustris gen. nov., sp. nov., a new
methane-oxidizing acidophilic bacterium from peat bogs, representing a
novel subtype of serine-pathway methanotrophs. Int. J. Syst. Evol. Microbiol.
50:955–969.
5. Dedysh, S. N., M. Derakshani, and W. Liesack. 2001. Detection and
enumeration of methanotrophs in acidic Sphagnum peat by 16S rRNA
fluorescence in situ hybridization, including the use of newly developed
oligonucleotide probes for Methylocella palustris. Appl. Environ. Microbiol.
67:4850–4857.
6. Dedysh, S. N., et al. 2004. Methylocella tundrae sp. nov., a novel methanotrophic
bacterium from acidic tundra peatlands. Int. J. Syst. Evol. Microbiol.
54:151–156.
7. Dedysh, S. N., C. Knief, and P. F. Dunfield. 2005. Methylocella species are
facultatively methanotrophic. J. Bacteriol. 187:4665–4670.
8. Drake, H. L., K. Ku¨sel, and C. C. Matthies. 2006. Acetogenic prokaryotes, p.
354–420. In A. Balows, H. G. Tru¨per, M. Dworkin, W. Harder, and K. H.
Schleifer (ed.), The prokaryotes. Springer, New York, NY.
9. Duddleston, K. N., and M. A. Kinney. 2002. Anaerobic microbial biogeochemistry
in a northern bog: acetate as a dominant metabolic end product.
Global Biogeochem. Cycles 16:1–9.
10. Dunfield, P. F., V. N. Khmelenina, N. E. Suzina, Y. A. Trotsenko, and S. N.
Dedysh. 2003. Methylocella silvestris sp. nov., a novel methanotroph isolated
from an acidic forest cambisol. Int. J. Syst. Evol. Microbiol. 53:1231–1239.
11. Im, J., and J. D. Semrau. 2011. Pollutant degradation by a Methylocystis
strain SB2 grown on ethanol: bioremediation via facultative methanotrophy.
FEMS Microbiol. Lett. doi:10.1111/j.1574-6968.2011.02249.x.
12. Ku¨sel, K., and H. L. Drake. 1995. Effects of environmental parameters on
the formation and turnover of acetate by forest soils. Appl. Environ. Microbiol.
61:3667–3675.
13. Ku¨sel, K., and H. L. Drake. 1999. Microbial turnover of low molecular
organic acids during leaf litter decomposition. Soil Biol. Biochem. 31:107–
118.
14. Liu, F., and R. Conrad. 2010. Thermoanaerobacteriaceae oxidize acetate in
methanogenic rice field soil at 50°C. Environ. Microbiol. 12:2341–2354.
15. McDonald, I. R., H. Uchiyama, S. Kambe, O. Yagi, and J. C. Murrell. 1997.
The soluble methane monooxygenase gene cluster of the trichloroethylenedegrading
methanotroph Methylocystis sp. strain M. Appl. Environ. Microbiol.
63:1898–1904.
16. Radajewski, S., P. Ineson, N. R. Parekh, and M. J. Murrell. 2000. Stableisotope
probing as a tool in microbial ecology. Nature 403:646–649.
17. Rahman, M. T., et al. 2010. Environmental distribution and abundance of
the facultative methanotroph Methylocella. ISME J. [Epub ahead of print.]
doi:10.1038/ismej.2010.190.
18. Semrau, J. D., A. A. DiSpirito, and S. Yoon. 2010. Methanotrophs and
copper. FEMS Microbiol. Rev. 34:496–531.
19. Theisen, A. R., et al. 2005. Regulation of methane oxidation in the facultative
methanotroph Methylocella silvestris BL2. Mol. Microbiol. 58:682–692.
20. Trotsenko, Y. A., and J. C. Murrell. 2008. Metabolic aspects of aerobic
obligate methanotrophy. Adv. Appl. Microbiol. 63:183–229.
21. Vorobev, A. V., et al. 2010. Methyloferula stellata gen. nov., sp. nov., an
acidophilic, obligately methanotrophic bacterium possessing only a soluble
methane monooxygenase. Int. J. Syst. Evol. Microbiol. doi:10.1099/
ijs.0.028118-0.