Quinn, J. (Janet), Malakasi, Panagiota, Smith, Deborah A., Cheetham, Jill, Buck, Vicky, Millar, Jonathan B. A. and Morgan, Brian A. (2011) Two-component mediated peroxide sensing and signal transduction in fission yeast. Antioxidants & Redox Signaling, Volume 15 (Number 1). pp. 153-165. doi:10.1089/ars.2010.3345 ISSN 1523-0864.

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Abstract

Two-component related proteins play a major role in regulating the oxidative stress response in the fission yeast, Schizosaccharomyces pombe. For example, the peroxide-sensing Mak2 and Mak3 histidine kinases regulate H(2)O(2)-induced activation of the Sty1 stress-activated protein kinase pathway, and the Skn7-related response regulator transcription factor, Prr1, is essential for activation of the core oxidative stress response genes. Here, we investigate the mechanism by which the S. pombe two-component system senses H(2)O(2), and the potential role of two-component signaling in the regulation of Prr1. Significantly, we demonstrate that PAS and GAF domains present in the Mak2 histidine kinase are essential for redox-sensing and activation of Sty1. In addition, we find that Prr1 is required for the transcriptional response to a wide range of H(2)O(2) concentrations and, furthermore, that two-component regulation of Prr1 is specifically required for the response of cells to high levels of H(2)O(2). Significantly, this provides the first demonstration that the conserved two-component phosphorylation site on Skn7-related proteins influences resistance to oxidative stress and oxidative stress-induced gene expression. Collectively, these data provide new insights into the two-component mediated sensing and signaling mechanisms underlying the response of S. pombe to oxidative stress. Antioxid. Redox Signal. 15, 153-165.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany Q Science > QP Physiology
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Fission (Biology), Schizosaccharomyces pombe, Cellular signal transduction
Journal or Publication Title:	Antioxidants & Redox Signaling
Publisher:	Antioxidants & Redox Signaling
ISSN:	1523-0864
Official Date:	2011
Dates:	Date Event 2011 UNSPECIFIED
Volume:	Volume 15
Number:	Number 1
Page Range:	pp. 153-165
DOI:	10.1089/ars.2010.3345
Status:	Peer Reviewed
Publication Status:	Published
Funder:	Medical Research Council (Great Britain) (MRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)

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