Two-component mediated peroxide sensing and signal transduction in fission yeast
Quinn, J. (Janet), Malakasi, Panagiota, Smith, Deborah A., Cheetham, Jill, Buck, Vicky, Millar, Jonathan B. A. and Morgan, Brian A.. (2011) Two-component mediated peroxide sensing and signal transduction in fission yeast. Antioxidants & Redox Signaling, Volume 15 (Number 1). pp. 153-165. ISSN 1523-0864Full text not available from this repository.
Official URL: http://dx.doi.org/10.1089/ars.2010.3345
Two-component related proteins play a major role in regulating the oxidative stress response in the fission yeast, Schizosaccharomyces pombe. For example, the peroxide-sensing Mak2 and Mak3 histidine kinases regulate H(2)O(2)-induced activation of the Sty1 stress-activated protein kinase pathway, and the Skn7-related response regulator transcription factor, Prr1, is essential for activation of the core oxidative stress response genes. Here, we investigate the mechanism by which the S. pombe two-component system senses H(2)O(2), and the potential role of two-component signaling in the regulation of Prr1. Significantly, we demonstrate that PAS and GAF domains present in the Mak2 histidine kinase are essential for redox-sensing and activation of Sty1. In addition, we find that Prr1 is required for the transcriptional response to a wide range of H(2)O(2) concentrations and, furthermore, that two-component regulation of Prr1 is specifically required for the response of cells to high levels of H(2)O(2). Significantly, this provides the first demonstration that the conserved two-component phosphorylation site on Skn7-related proteins influences resistance to oxidative stress and oxidative stress-induced gene expression. Collectively, these data provide new insights into the two-component mediated sensing and signaling mechanisms underlying the response of S. pombe to oxidative stress. Antioxid. Redox Signal. 15, 153-165.
|Item Type:||Journal Article|
|Subjects:||Q Science > QK Botany
Q Science > QP Physiology
|Divisions:||Faculty of Science > Life Sciences (2010- )|
|Library of Congress Subject Headings (LCSH):||Fission (Biology), Schizosaccharomyces pombe, Cellular signal transduction|
|Journal or Publication Title:||Antioxidants & Redox Signaling|
|Page Range:||pp. 153-165|
|Funder:||Medical Research Council (Great Britain) (MRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)|
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