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Recombinants between Deformed wing virus and Varroa destructor virus-1 may prevail in Varroa destructor-infested honeybee colonies

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Moore, Jonathan D., Jironkin, Aleksey, Chandler, Dave, Burroughs, Nigel John, Evans, D. J. (David J.) and Ryabov, Eugene . (2011) Recombinants between Deformed wing virus and Varroa destructor virus-1 may prevail in Varroa destructor-infested honeybee colonies. Journal of General Virology, Vol.92 . pp. 156-161. ISSN 0022-1317

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Official URL: http://dx.doi.org/10.1099/vir.0.025965-0

Abstract

We have used high-throughput Illumina sequencing to identify novel recombinants between deformed wing virus (DWV) and Varroa destructor virus-1 (VDV-1), which accumulate to higher levels than DWV in both honeybees and Varroa destructor mites. The recombinants, VDV-1VVD and VDV-1DVD, exhibit crossovers between the 5’-untranslated region (5’-UTR), and/or the regions encoding the structural (capsid) and non-structural viral proteins. This implies the genomes are modular and that each region may evolve independently, as demonstrated in human enteroviruses. Individual honeybee pupae were infected with a mixture of observed recombinants and DWV. The strong correlation between VDV-1DVD levels in honeybee pupae and the associated mites was observed, suggesting that this recombinant, with a DWV-derived 5’-UTR and non-structural protein region flanking VDV- 1-derived capsid encoding region, is better adapted to transmission between V. destructor and honeybees than the parental DWV or a recombinant bearing the VDV-1-derived 5’-UTR (VDV-1VVD).

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology > QR355 Virology
Divisions:	Faculty of Science > Life Sciences (2010- ) Faculty of Science > Mathematics Faculty of Science > Centre for Systems Biology
Library of Congress Subject Headings (LCSH):	Varroa, Picornaviruses, Recombinant viruses, Honeybee -- Diseases
Journal or Publication Title:	Journal of General Virology
Publisher:	Society for General Microbiology
ISSN:	0022-1317
Date:	2011
Volume:	Vol.92
Page Range:	pp. 156-161
Identification Number:	10.1099/vir.0.025965-0
Status:	Peer Reviewed
Access rights to Published version:	Restricted or Subscription Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Great Britain. Dept. for Environment, Food & Rural Affairs (DEFRA), Medical Research Council (Great Britain) (MRC)
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URI:	http://wrap.warwick.ac.uk/id/eprint/4141