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Baculovirus resistance in codling moth (Cydia pomonella L.) caused by early block of virus replication
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Asser-Kaiser, Sabine, Radtke, Pit, El-Salamouny, Said, Winstanley, Doreen and Jehle, Johannes A. (2011) Baculovirus resistance in codling moth (Cydia pomonella L.) caused by early block of virus replication. Virology, Volume 410 (Number 2). pp. 360-367. doi:10.1016/j.virol.2010.11.021 ISSN 0042-6822.
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Official URL: http://dx.doi.org/10.1016/j.virol.2010.11.021
Abstract
An up to 10,000-fold resistance against the biocontrol agent Cydia pomonella granulovirus (CpGV) was observed in field populations of codling moth, C pomonella, in Europe. Following different experimental approaches, a modified peritrophic membrane, a modified midgut receptor, or a change of the innate immune response could be excluded as possible resistance mechanisms. When CpGV replication was traced by quantitative PCR in different tissues of susceptible and resistant insects after oral and intra-hemocoelic infection, no virus replication could be detected in any of the tissues of resistant insects, suggesting a systemic block prior to viral DNA replication. This conclusion was corroborated by fluorescence microscopy using a modified CpGV (bacCpGV(hsp-eGFP)) carrying enhanced green fluorescent gene (eGFP), which showed that infection in resistant insects did not spread. In conclusion, the different lines of evidence indicate that CpGV can enter but not replicate in the cells of resistant codling moth larvae. (C) 2010 Elsevier Inc. All rights reserved.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QR Microbiology > QR355 Virology S Agriculture > SB Plant culture |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) | ||||
| Library of Congress Subject Headings (LCSH): | Baculoviruses, Codling moth -- Diseases, Insects -- Virus diseases, Green fluorescent protein | ||||
| Journal or Publication Title: | Virology | ||||
| Publisher: | Academic Press | ||||
| ISSN: | 0042-6822 | ||||
| Official Date: | 20 February 2011 | ||||
| Dates: |
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| Volume: | Volume 410 | ||||
| Number: | Number 2 | ||||
| Page Range: | pp. 360-367 | ||||
| DOI: | 10.1016/j.virol.2010.11.021 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Bundesanstalt für Landwirtschaft und Ernährung (Germany) [Federal Agency for Agriculture and Food] (BLE), Boehringer Ingelheim Fonds | ||||
| Grant number: | Federal Agency for Agriculture and Food (BLE) of Germany (050EO23/1) |
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