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Baculovirus resistance in codling moth (Cydia pomonella L.) caused by early block of virus replication

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Asser-Kaiser, Sabine, Radtke, Pit, El-Salamouny, Said, Winstanley, Doreen and Jehle, Johannes A. (2011) Baculovirus resistance in codling moth (Cydia pomonella L.) caused by early block of virus replication. Virology, Volume 410 (Number 2). pp. 360-367. doi:10.1016/j.virol.2010.11.021 ISSN 0042-6822.

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Official URL: http://dx.doi.org/10.1016/j.virol.2010.11.021

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Abstract

An up to 10,000-fold resistance against the biocontrol agent Cydia pomonella granulovirus (CpGV) was observed in field populations of codling moth, C pomonella, in Europe. Following different experimental approaches, a modified peritrophic membrane, a modified midgut receptor, or a change of the innate immune response could be excluded as possible resistance mechanisms. When CpGV replication was traced by quantitative PCR in different tissues of susceptible and resistant insects after oral and intra-hemocoelic infection, no virus replication could be detected in any of the tissues of resistant insects, suggesting a systemic block prior to viral DNA replication. This conclusion was corroborated by fluorescence microscopy using a modified CpGV (bacCpGV(hsp-eGFP)) carrying enhanced green fluorescent gene (eGFP), which showed that infection in resistant insects did not spread. In conclusion, the different lines of evidence indicate that CpGV can enter but not replicate in the cells of resistant codling moth larvae. (C) 2010 Elsevier Inc. All rights reserved.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology > QR355 Virology
S Agriculture > SB Plant culture
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH): Baculoviruses, Codling moth -- Diseases, Insects -- Virus diseases, Green fluorescent protein
Journal or Publication Title: Virology
Publisher: Academic Press
ISSN: 0042-6822
Official Date: 20 February 2011
Dates:
DateEvent
20 February 2011Published
Volume: Volume 410
Number: Number 2
Page Range: pp. 360-367
DOI: 10.1016/j.virol.2010.11.021
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Bundesanstalt für Landwirtschaft und Ernährung (Germany) [Federal Agency for Agriculture and Food] (BLE), Boehringer Ingelheim Fonds
Grant number: Federal Agency for Agriculture and Food (BLE) of Germany (050EO23/1)

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