Temporal repression of core circadian genes is mediated through EARLY FLOWERING 3 in Arabidopsis
Dixon, Laura E., Knox, Kirsten, Kozma-Bognar, Laszlo, Southern, Megan M., Pokhilko, Alexandra and Millar, Andrew J.. (2011) Temporal repression of core circadian genes is mediated through EARLY FLOWERING 3 in Arabidopsis. Current Biology, Vol.21 (No.2). pp. 120-125. ISSN 0960-9822Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.cub.2010.12.013
The circadian clock provides robust, similar to 24 hr biological rhythms throughout the eukaryotes. The clock gene circuit in plants comprises interlocking transcriptional feedback loops, reviewed in , whereby the morning-expressed transcription factors CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) repress the expression of evening genes, notably TIMING OF CAB EXPRESSION 1 (TOC1). EARLY FLOWERING 3 (ELF3) has been implicated as a repressor of light signaling to the clock [2, 3] and, paradoxically, as an activator of the light-induced genes CCA1 and LHY [4, 5]. We use cca1-11 lhy-21 elf3-4 plants to separate the repressive function of ELF3 from its downstream targets CCA1 and LHY. We further demonstrate that ELF3 associates physically with the promoter of PSEUDO-RESPONSE REGULATOR 9 (PRR9), a repressor of CCA1 and LHY expression, in a time-dependent fashion. The repressive function of ELF3 is thus consistent with indirect activation of LHY and CCA1, in a double-negative connection via a direct ELF3 target, PRR9. This mechanism reconciles the functions of ELF3 in the clock network during the night and points to further effects of ELF3 during the day.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Journal or Publication Title:||Current Biology|
|Official Date:||25 January 2011|
|Page Range:||pp. 120-125|
|Funder:||Biotechnology and Biological Sciences Research Council (BBSRC) , Hungarian Scientific Research Fund (OTKA), Magyar Tudományos Akadémia [Hungarian Academy of Sciences], Engineering and Physical Sciences Research Council (EPSRC)|
|Grant number:||BB/E015263/1 (BBSRC), OTKA-73362 (OTKA), BB/D019621/1 (EPSRC)|
Actions (login required)