Labor and inflammation increase the expression of oxytocin receptor in human amnion
Terzidou, V., Blanks, Andrew M., Kim, S. H., Thornton, Steve and Bennett, P. R.. (2011) Labor and inflammation increase the expression of oxytocin receptor in human amnion. Biology of Reproduction, Vol.84 (No.3). pp. 546-552. ISSN 0006-3363Full text not available from this repository.
Official URL: http://dx.doi.org/10.1095/biolreprod.110.086785
The oxytocin/oxytocin receptor (OXT/OXTR) system plays an important role in the regulation of parturition. The amnion is a major source of prostaglandins and inflammatory cytokine synthesis, which increase both before and during labor. Amnion is a noncontractile tissue; therefore, the role played by OXT/OXTR in this tissue will be fundamentally different from the role played in myometrial contractions. In the present study, we demonstrate increased OXTR mRNA and protein concentrations in human amnion epithelial cells associated with the onset of labor. We show that incubation of primary human amnion epithelial cells with IL1B results in a rapid, transient up-regulation of OXTR mRNA expression, which peaks in prelabor samples after 6 h. Incubation of prelabor amnion epithelial cells with OXT results in a marked increase of prostaglandin E(2) synthesis, and we demonstrate that OXT activates the extracellular signal-regulated protein kinase signal transduction pathway to stimulate up-regulation of cyclo-oxygenase 2 in human amnion epithelial cells. The increased ability of human amnion to produce prostaglandins in response to OXT treatment suggests a complementary role for the OXT/OXTR system in the activation of human amnion and the onset of labor.
|Item Type:||Journal Article|
|Subjects:||R Medicine > R Medicine (General)|
|Divisions:||Faculty of Medicine > Warwick Medical School > Clinical Sciences Research Institute (CSRI)
Faculty of Medicine > Warwick Medical School > Reproductive Health
Faculty of Medicine > Warwick Medical School
|Journal or Publication Title:||Biology of Reproduction|
|Publisher:||Society for the Study of Reproduction|
|Page Range:||pp. 546-552|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Action Medical Research Project , National Institute for Health, Biomedical Research Centre|
|Grant number:||SP4454 (Action Medical Research Project), DSRR_NG0122/2009 (NIH)|
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