Terzidou, Vasso, Blanks, Andrew M., Kim, Sung Hye, Thornton, Steven and Bennett, Phillip R.. (2011) Labor and inflammation increase the expression of oxytocin receptor in human amnion. Biology of Reproduction, Volume 84 (Number 3). pp. 546-552. ISSN 0006-3363

Full text not available from this repository.

Abstract

The oxytocin/oxytocin receptor (OXT/OXTR) system plays an important role in the regulation of parturition. The amnion is a major source of prostaglandins and inflammatory cytokine synthesis, which increase both before and during labor. Amnion is a noncontractile tissue; therefore, the role played by OXT/OXTR in this tissue will be fundamentally different from the role played in myometrial contractions. In the present study, we demonstrate increased OXTR mRNA and protein concentrations in human amnion epithelial cells associated with the onset of labor. We show that incubation of primary human amnion epithelial cells with IL1B results in a rapid, transient up-regulation of OXTR mRNA expression, which peaks in prelabor samples after 6 h. Incubation of prelabor amnion epithelial cells with OXT results in a marked increase of prostaglandin E(2) synthesis, and we demonstrate that OXT activates the extracellular signal-regulated protein kinase signal transduction pathway to stimulate up-regulation of cyclo-oxygenase 2 in human amnion epithelial cells. The increased ability of human amnion to produce prostaglandins in response to OXT treatment suggests a complementary role for the OXT/OXTR system in the activation of human amnion and the onset of labor.

[error in script] [error in script]

Item Type:	Journal Article
Subjects:	Q Science > QM Human anatomy Q Science > QP Physiology
Divisions:	Faculty of Medicine > Warwick Medical School > Clinical Sciences Research Institute (CSRI) Faculty of Medicine > Warwick Medical School > Reproductive Health Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Oxytocin -- Receptors, Amnion, Inflammation, Prostaglandins, Parturition
Journal or Publication Title:	Biology of Reproduction
Publisher:	Society for the Study of Reproduction
ISSN:	0006-3363
Date:	March 2011
Volume:	Volume 84
Number:	Number 3
Page Range:	pp. 546-552
Identification Number:	10.1095/biolreprod.110.086785
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Action Medical Research (AMR), National Institute for Health Research (Great Britain) (NIHR)
Grant number:	SP4454 (AMR), DSRR_NG0122/2009 (NIHR)
References:	1. Kimura T, Tanizawa O, Mori K, Brownstein MJ, Okayama H. Structure and expression of a human oxytocin receptor. Nature 1992; 356:526–529 [published erratum appears in Nature 1992; 357:176]. 2. Hinko A, Soloff MS. Characterization of oxytocin receptors in rabbit amnion involved in the production of prostaglandin E2. Endocrinology 1992; 130:3547–3553. 3. Benedetto MT, De Cicco F, Rossiello F, Nicosia AL, Lupi G, Dell’Acqua S. Oxytocin receptor in human fetal membranes at term and during labor. J Steroid Biochem 1990; 35:205–208. 4. Van Meir CA, Ramirez MM, Matthews SG, Calder AA, Keirse MJ, Challis JR. Chorionic prostaglandin catabolism is decreased in the lower uterine segment with term labor. Placenta 1997; 18:109–114. 5. Roseblade CK, Sullivan MH, Khan H, Lumb MR, Elder MG. Limited transfer of prostaglandin E2 across the fetal membrane before and after labor. Acta Obstet Gynecol Scand 1990; 69:399–403. 6. Johnston PC, Greer IA, Brooks AN. The transfer of prostaglandin E2 across ovine fetal membranes in vivo. J Soc Gynecol Investig 1996; 3: 303–308. 7. Allport VC, Pieber D, Slater DM, Newton R, White JO, Bennett PR. Human labor is associated with nuclear factor-kappaB activity which mediates cyclo-oxygenase-2 expression and is involved with the ‘‘functional progesterone withdrawal.’’ Mol Hum Reprod 2001; 7:581– 586. 8. Slater DM, Dennes WJ, Campa JS, Poston L, Bennett PR. Expression of cyclo-oxygenase types-1 and -2 in human myometrium throughout pregnancy. Mol Hum Reprod 1999; 5:880–884. 9. Sawdy RJ, Slater DM, Dennes WJ, Sullivan MH, Bennett PR. The roles of the cyclo-oxygenases types one and two in prostaglandin synthesis in human fetal membranes at term. Placenta 2000; 21:54–57. 10. Slater D, Dennes W, Sawdy R, Allport V, Bennett P. Expression of cyclooxygenase types-1 and -2 in human fetal membranes throughout pregnancy. J Mol Endocrinol 1999; 22:125–130. 11. Zakar T, Olson DM, Teixeira FJ, Hirst JJ. Regulation of prostaglandin endoperoxide H2 synthase in term human gestational tissues. Acta Physiol Hung 1996; 84:109–118. 12. Lindstrom TM, Bennett PR. The role of nuclear factor kappa B in human labor. Reproduction 2005; 130:569–581. 13. Lim S, Khanjani S, Terzidou V, Lee YS, Teoh T, Bennett PR. Wholegenome array and si-RNA investigation of the function of NF-kB in human amnion epithelial cells. Reprod Sci 2008; 15:260. 14. Anderson NG, Maller JL, Tonks NK, Sturgill TW. Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase. Nature 1990; 343:651–653. 15. Ruzycky AL. Down-regulation of the mitogen-activated protein kinase cascade immediately before parturition in the rat myometrium. J Soc Gynecol Investig 1998; 5:304–310. 16. Li Y, Je HD, Malek S, Morgan KG. ERK1/2-mediated phosphorylation of myometrial caldesmon during pregnancy and labor. Am J Physiol Regul Integr Comp Physiol 2003; 284:R192–R199. 17. Otun HA, MacDougall MW, Bailey J, Europe-Finner GN, Robson SC. Spatial and temporal expression of the myometrial mitogen-activated protein kinases p38 and ERK1/2 in the human uterus during pregnancy and labor. J Soc Gynecol Investig 2005; 12:185–190. 18. Lin LL, Wartmann M, Lin AY, Knopf JL, Seth A, Davis RJ. cPLA2 is phosphorylated and activated by MAP kinase. Cell 1993; 72:269–278. 19. Lin LL, Lin AY, Knopf JL. Cytosolic phospholipase A2 is coupled to hormonally regulated release of arachidonic acid. Proc Natl Acad Sci U S A 1992; 89:6147–6151. 20. Bennett PR, Rose MP, Myatt L, Elder MG. Preterm labor: stimulation of arachidonic acid metabolism in human amnion cells by bacterial products. Am J Obstet Gynecol 1987; 156:649–655. 21. Pohnke Y, Kempf R, Gellersen B. CCAAT/enhancer-binding proteins are mediators in the protein kinase A-dependent activation of the decidual prolactin promoter. J Biol Chem 1999; 274:24808–24818. 22. Chibbar R, Miller FD, Mitchell BF. Synthesis of oxytocin in amnion, chorion, and decidua may influence the timing of human parturition. J Clin Invest 1993; 91:185–192. 23. Blanks AM, Vatish M, Allen MJ, Ladds G, de Wit NC, Slater DM, Thornton S. Paracrine oxytocin and estradiol demonstrate a spatial increase in human intrauterine tissues with labor. J Clin Endocrinol Metab 2003; 88:3392–3400. 24. Rauk PN, Friebe-Hoffmann U. Interleukin-1beta down-regulates the oxytocin receptor in cultured uterine smooth muscle cells. Am J Reprod Immunol 2000; 43:85–91. 25. Mitchell BF, Schmid B. Oxytocin and its receptor in the process of parturition. J Soc Gynecol Investig 2001; 8:122–133. 26. Schmid B, Wong S, Mitchell BF. Transcriptional regulation of oxytocin receptor by interleukin-1beta and interleukin-6. Endocrinology 2001; 142: 1380–1385. 27. Romero R, Mazor M, Brandt F, Sepulveda W, Avila C, Cotton DB, Dinarello CA. Interleukin-1alpha and interleukin-1beta in preterm and term human parturition. Am J Reprod Immunol 1992; 27:117–123. 28. Loudon JA, Elliott CL, Hills F, Bennett PR. Progesterone represses interleukin-8 and cyclo-oxygenase-2 in human lower segment fibroblast cells and amnion epithelial cells. Biol Reprod 2003; 69:331–337. 29. Gilroy DW, Saunders MA, Wu KK. COX-2 expression and cell cycle progression in human fibroblasts. Am J Physiol Cell Physiol 2001; 281: C188–C194. 30. Slater D, Allport V, Bennett P. Changes in the expression of the type-2 but not the type-1 cyclo-oxygenase enzyme in chorion-decidua with the onset of labor. Br J Obstet Gynaecol 1998; 105:745–748. 31. Bartlett SR, Sawdy R, Mann GE. Induction of cyclo-oxygenase-2 expression in human myometrial smooth muscle cells by interleukin- 1beta: involvement of p38 mitogen-activated protein kinase. J Physiol 1999; 520(pt 2):399–406. 32. Slater DM, Berger LC, Newton R, Moore GE, Bennett PR. Expression of cyclo-oxygenase types 1 and 2 in human fetal membranes at term. Am J Obstet Gynecol 1995; 172:77–82. 33. Slater D, Berger L, Newton R, Moore G, Bennett P. The relative abundance of type 1 to type 2 cyclo-oxygenase mRNA in human amnion at term. Biochem Biophys Res Commun 1994; 198:304–308. 34. Lee JS, Silvia WJ. Cellular mechanisms mediating the stimulation of ovine endometrial secretion of prostaglandin F2alpha in response to oxytocin: role of phospholipase A2. J Endocrinol 1994; 141:491–496. 35. Asselin E, Drolet P, Fortier MA. Cellular mechanisms involved during oxytocin-induced prostaglandin F2alpha production in endometrial epithelial cells in vitro: role of cyclo-oxygenase-2. Endocrinology 1997; 138: 4798–4805. 36. Burns PD, Tsai SJ, Wiltbank MC, Hayes SH, Graf GA, Silvia WJ. Effect of oxytocin on concentrations of prostaglandin H synthase-2 mRNA in ovine endometrial tissue in vivo. Endocrinology 1997; 138:5637–5640. 37. Jeng YJ, Liebenthal D, Strakova Z, Ives KL, Hellmich MR, Soloff MS. Complementary mechanisms of enhanced oxytocin-stimulated prostaglandin E2 synthesis in rabbit amnion at the end of gestation. Endocrinology 2000; 141:4136–4145. 38. Dawood MY, Wang CF, Gupta R, Fuchs F. Fetal contribution to oxytocin in human labor. Obstet Gynecol 1978; 52:205–209. 39. Morel G, Pechoux C, Raccurt M, Zingg HH. Intrauterine oxytocin system. Compartmental distribution of oxytocin and oxytocin receptors in rat endometrial epithelium. Cell Tissue Res 2001; 304:377–382. 40. Strakova Z, Copland JA, Lolait SJ, Soloff MS. ERK2 mediates oxytocinstimulated PGE2 synthesis. Am J Physiol 1998; 274:E634–E641.
URI:	http://wrap.warwick.ac.uk/id/eprint/41633

Data sourced from Thomson Reuters' Web of Knowledge

Request changes to a record

Actions (login required)

View Item