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Active methylotrophs in the sediments of Lonar Lake, a saline and alkaline ecosystem formed by meteor impact
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Antony, Chakkiath Paul, Kumaresan, Deepak, Ferrando, Lucia, Boden, Rich, Moussard, Hélène, Scavino, Ana Fernández, Shouche, Yogesh S and Murrell, J. C. (J. Colin) (2010) Active methylotrophs in the sediments of Lonar Lake, a saline and alkaline ecosystem formed by meteor impact. ISME Journal, Volume 4 (Number 11). pp. 1470-1480. doi:10.1038/ismej.2010.70 ISSN 1751-7362.
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Official URL: http://dx.doi.org/10.1038/ismej.2010.70
Abstract
Lonar Lake is a unique saline and alkaline ecosystem formed by meteor impact in the Deccan basalts in India around 52 000 years ago. To investigate the role of methylotrophy in the cycling of carbon in this unusual environment, stable-isotope probing (SIP) was carried out using the one-carbon compounds methane, methanol and methylamine. Denaturing gradient gel electrophoresis fingerprinting analyses performed with heavy (13)C-labelled DNA retrieved from sediment microcosms confirmed the enrichment and labelling of active methylotrophic communities. Clone libraries were constructed using PCR primers targeting 16S rRNA genes and functional genes. Methylomicrobium, Methylophaga and Bacillus spp. were identified as the predominant active methylotrophs in methane, methanol and methylamine SIP microcosms, respectively. Absence of mauA gene amplification in the methylamine SIP heavy fraction also indicated that methylamine metabolism in Lonar Lake sediments may not be mediated by the methylamine dehydrogenase enzyme pathway. Many gene sequences retrieved in this study were not affiliated with extant methanotrophs or methylotrophs. These sequences may represent hitherto uncharacterized novel methylotrophs or heterotrophic organisms that may have been cross-feeding on methylotrophic metabolites or biomass. This study represents an essential first step towards understanding the relevance of methylotrophy in the soda lake sediments of an unusual impact crater structure. The ISME Journal (2010) 4, 1470-1480; doi: 10.1038/ismej.2010.70; published online 17 June 2010
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
| Library of Congress Subject Headings (LCSH): | Methylotrophic microorganisms, Lonar Lake (India), Molecular probes, Stable isotope tracers, Carbon compounds, Biotic communities, Salt lake ecology, Alkali lakes | ||||
| Journal or Publication Title: | ISME Journal | ||||
| Publisher: | Nature Publishing Group | ||||
| ISSN: | 1751-7362 | ||||
| Official Date: | November 2010 | ||||
| Dates: |
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| Volume: | Volume 4 | ||||
| Number: | Number 11 | ||||
| Page Range: | pp. 1470-1480 | ||||
| DOI: | 10.1038/ismej.2010.70 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Funder: | UK-India Education and Research Initiative (UKIERI), Natural Environment Research Council (Great Britain) (NERC), Indian Council of Medical Research (ICMR), Programa de Desarrollo Tecnológico (Uruguay) (PDT), Universidad de la República (Uruguay). Comisión Sectorial de Investigación Científica (CSIC) | ||||
| Grant number: | SA07-061 (UKIERI) |
Data sourced from Thomson Reuters' Web of Knowledge
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