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Elucidating mechanistic principles underpinning eukaryotic translation initiation using quantitative fluorescence methods

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Stevenson, Abigail L., Juanes, Pedro P. and McCarthy, John E. G. (2010) Elucidating mechanistic principles underpinning eukaryotic translation initiation using quantitative fluorescence methods. Biochemical Society Transactions, Volume 38 (Number 6). pp. 1587-1592. doi:10.1042/BST0381587

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Official URL: http://dx.doi.org/10.1042/BST0381587

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Abstract

Eukaryotic translation initiation is an intricate process involving at least 11 formally classified eIFs (eukaryotic initiation factors) which together with the ribosome comprise one of the largest molecular machines in the cell Studying such huge macromolecular complexes presents many challenges which cannot readily be overcome by traditional molecular and structural methods Increasingly novel quantitative techniques are being used to further dissect such complex assembly pathways One area of methodology involves the labelling of ribosomal subunits and/or eIFs with fluorophores and the use of techniques such as FRET (Forster resonance energy transfer) and FA (fluorescence anisotropy) The applicability of such techniques in such a complex system has been greatly enhanced by recent methodological developments In the present mini review we introduce these quantitative fluorescence methods and discuss the impact they are beginning to have on the field

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Eukaryotic cells, Genetic translation, Fluorescence microscopy, Macromolecules
Journal or Publication Title: Biochemical Society Transactions
Publisher: Portland Press Ltd
ISSN: 0300-5127
Official Date: 2010
Dates:
DateEvent
2010Published
Volume: Volume 38
Number: Number 6
Page Range: pp. 1587-1592
DOI: 10.1042/BST0381587
Status: Peer Reviewed
Publication Status: Published
Funder: Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)
Grant number: BB/E024181/1 and BB/E011888/1 (BBSRC)

Data sourced from Thomson Reuters' Web of Knowledge

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