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Elucidating mechanistic principles underpinning eukaryotic translation initiation using quantitative fluorescence methods
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Stevenson, Abigail L., Juanes, Pedro P. and McCarthy, John E. G. (2010) Elucidating mechanistic principles underpinning eukaryotic translation initiation using quantitative fluorescence methods. Biochemical Society Transactions, Volume 38 (Number 6). pp. 1587-1592. doi:10.1042/BST0381587
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Official URL: http://dx.doi.org/10.1042/BST0381587
Abstract
Eukaryotic translation initiation is an intricate process involving at least 11 formally classified eIFs (eukaryotic initiation factors) which together with the ribosome comprise one of the largest molecular machines in the cell Studying such huge macromolecular complexes presents many challenges which cannot readily be overcome by traditional molecular and structural methods Increasingly novel quantitative techniques are being used to further dissect such complex assembly pathways One area of methodology involves the labelling of ribosomal subunits and/or eIFs with fluorophores and the use of techniques such as FRET (Forster resonance energy transfer) and FA (fluorescence anisotropy) The applicability of such techniques in such a complex system has been greatly enhanced by recent methodological developments In the present mini review we introduce these quantitative fluorescence methods and discuss the impact they are beginning to have on the field
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology | ||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||
| Library of Congress Subject Headings (LCSH): | Eukaryotic cells, Genetic translation, Fluorescence microscopy, Macromolecules | ||||
| Journal or Publication Title: | Biochemical Society Transactions | ||||
| Publisher: | Portland Press Ltd | ||||
| ISSN: | 0300-5127 | ||||
| Official Date: | 2010 | ||||
| Dates: |
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| Volume: | Volume 38 | ||||
| Number: | Number 6 | ||||
| Page Range: | pp. 1587-1592 | ||||
| DOI: | 10.1042/BST0381587 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||
| Grant number: | BB/E024181/1 and BB/E011888/1 (BBSRC) |
Data sourced from Thomson Reuters' Web of Knowledge
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