Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Aberrant response to commensal Bacteroides thetaiotaomicron in Crohn's disease : an ex vivo human organ culture study

Tools
- Tools
+ Tools

Edwards, L. A., Lucas, M., Edwards, E. A., Torrente, F., Heuschkel, R .B., Klein, N. J., Murch, Simon, Bajaj-Elliott, M. and Phillips, A .D. (2010) Aberrant response to commensal Bacteroides thetaiotaomicron in Crohn's disease : an ex vivo human organ culture study. Inflammatory Bowel Diseases, Vol.17 (No.5). pp. 1201-1208. doi:10.1002/ibd.21501

Research output not available from this repository, contact author.
Official URL: http://dx.doi.org/10.1002/ibd.21501

Request Changes to record.

Abstract

Background: Human ex vivo evidence indicating that an inappropriate immune response(s) to nonpathogenic bacteria contributes to disease pathogenesis in pediatric Crohn's disease (CD) is limited. The aim of the present study was to compare and contrast the early innate immune response of pediatric "healthy'' versus CD mucosa to pathogenic, probiotic, and commensal bacteria.

Methods: "Healthy control'' and CD pediatric mucosal biopsies (terminal ileum and transverse colon) were cocultured for 8 hours with E. coli O42, Lactobacillus GG (LGG), Bacteroides thetaiotaomicron (B. theta), or stimulated with interleukin (IL)-1 beta (positive control). Matched nonstimulated biopsies served as experimental controls. IL-8 was the immune marker of choice. IL-8 mRNA and protein levels were quantified by quantitative polymerase chain reaction and sandwich enzyme-linked immunosorbent assay, respectively.

Results: IL-8 secretion was observed when control, ileal biopsies were exposed to pathogenic O42 and probiotic LGG, with no response noted to commensal B. theta. In comparison, Crohn's ileal biopsies showed impaired ability to induce IL-8 in response to O42 and LGG. Control colonic tissue showed a limited response to O42 or B. theta and LGG significantly reduced IL-8 secretion. Unlike control tissue, however, Crohn's ileal and colonic tissue did respond to B. theta, with more enhanced expression in the colon.

Conclusions: We provide the first ex vivo data to support the notion that aberrant mucosal recognition of commensal bacteria may contribute to pediatric CD. While IL-8 responses to O42 and LGG varied with disease status and anatomical location, B. theta consistently induced significant IL-8 both in ileal and colonic CD tissue, which was not seen in control, healthy tissue. (Inflamm Bowel Dis 2011;17:1201-1208)

Item Type: Journal Article
Divisions: Faculty of Medicine > Warwick Medical School
Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine
Journal or Publication Title: Inflammatory Bowel Diseases
Publisher: John Wiley & Sons, Inc.
ISSN: 1078-0998
Official Date: 2010
Dates:
DateEvent
2010Published
Volume: Vol.17
Number: No.5
Page Range: pp. 1201-1208
DOI: 10.1002/ibd.21501
Status: Peer Reviewed
Publication Status: Published
Funder: Crohn's in Childhood Research Appeal (CICRA) charity

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us