Rittman, Martyn, Hoffmann, Søren V., Gilroy, Emma, Hicks, Matthew R., Finkenstädt, Bärbel and Rodger, Alison. (2012) Probing the structure of long DNA molecules in solution using synchrotron radiation linear dichroism. Physical Chemistry Chemical Physics, Vol.14 (No.1). pp. 353-366. ISSN 1463-9076

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Abstract

Linear dichroism (LD), a spectroscopic method for aligned samples, has been used with a synchrotron radiation source to reveal insights into the structure and stability of DNA with increasing salt concentrations (thus stabilizing the base pairing) and increasing temperature while remaining below the melting point (thus destabilizing the base pairing). Measurements have been made from 350 nm to 182 nm, and the spectral changes observed quantified using a Bayesian Markov chain Monte Carlo (MCMC) algorithm, which uses statistical methods to fit to experimental data. Based on literature H-D exchange experiments, we surmise that the cause of the spectral variations is the induction of transient single stranding of tracts in the DNA polymer, particularly those with significant content of the weaker AT base pairs. More detailed analysis of the LD data will require better nucleotide transition polarization assignments.

Item Type:	Journal Article
Divisions:	Faculty of Science > Chemistry
Journal or Publication Title:	Physical Chemistry Chemical Physics
Publisher:	Royal Society of Chemistry
ISSN:	1463-9076
Date:	2012
Volume:	Vol.14
Number:	No.1
Page Range:	pp. 353-366
Identification Number:	10.1039/c1cp22371b
Status:	Peer Reviewed
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/41938

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