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Self-assembly of Fmoc-tetrapeptides based on the RGDS cell adhesion motif
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Castelletto, V., Moulton, C. M., Cheng, G., Hamley, I. W., Hicks, Matthew R., Rodger, Alison, López-Pérez, Daniel E., Revilla-López, Guillermo and Alemán, Carlos (2011) Self-assembly of Fmoc-tetrapeptides based on the RGDS cell adhesion motif. Soft Matter, Vol.7 (No.24). 11405-11415 . doi:10.1039/c1sm06550e ISSN 1744-683X.
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Official URL: http://dx.doi.org/10.1039/c1sm06550e
Abstract
Self-assembly in aqueous solution has been investigated for two Fmoc [Fmoc - N-(fluorenyl)-9-methoxycarbonyl] tetrapeptides comprising the RGDS cell adhesion motif from fibronectin or the scrambled sequence GRDS. The hydrophobic Fmoc unit confers amphiphilicity on the molecules, and introduces aromatic stacking interactions. Circular dichroism and FTIR spectroscopy show that the self-assembly of both peptides at low concentration is dominated by interactions among Fmoc units, although Fmoc-GRDS shows beta-sheet features, at lower concentration than Fmoc-RGDS. Fibre X-ray diffraction indicates beta-sheet formation by both peptides at sufficiently high concentration. Strong alignment effects are revealed by linear dichroism experiments for Fmoc-GRDS. Cryo-TEM and small-angle X-ray scattering (SAXS) reveal that both samples form fibrils with a diameter of approximately 10 nm. Both Fmoc-tetrapeptides form self-supporting hydrogels at sufficiently high concentration. Dynamic shear rheometry enabled measurements of the moduli for the Fmoc-GRDS hydrogel, however syneresis was observed for the Fmoc-RGDS hydrogel which was significantly less stable to shear. Molecular dynamics computer simulations were carried out considering parallel and antiparallel beta-sheet configurations of systems containing 7 and 21 molecules of Fmoc-RGDS or Fmoc-GRDS, the results being analyzed in terms of both intermolecular structural parameters and energy contributions.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Journal or Publication Title: | Soft Matter | ||||
Publisher: | Royal Society of Chemistry | ||||
ISSN: | 1744-683X | ||||
Official Date: | 2011 | ||||
Dates: |
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Volume: | Vol.7 | ||||
Number: | No.24 | ||||
Page Range: | 11405-11415 | ||||
DOI: | 10.1039/c1sm06550e | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Engineering and Physical Sciences Research Council (EPSRC), Biotechnology and Biological Sciences Research Council (BBSRC), Generalitat de Catalunya | ||||
Grant number: | EP/F048114/1 EP/G026203/1 EP/F500378/1 (EPSRC) ; BB/F011199/1 ( (BBSRC) ; MAT2009-09138 2009SGR1208 (Generalitat de Catalunya0 |
Data sourced from Thomson Reuters' Web of Knowledge
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