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Some environmental factors affect growth and antibiotic production by the mycoparasite Coniothyrium minitans
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Tomprefa, Nicola, Hill, Robert, Whipps, J. M. and McQuilken, Mark (2011) Some environmental factors affect growth and antibiotic production by the mycoparasite Coniothyrium minitans. Biocontrol Science and Technology, Vol.21 (No.6). pp. 721-731. doi:10.1080/09583157.2011.575211 ISSN 0958-3157.
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Official URL: http://dx.doi.org/10.1080/09583157.2011.575211
Abstract
The effects of temperature and pH on growth and antibiotic production by three isolates of Coniothyrium minitans (Conio, Contans and IVT1), known to produce the macrolide antibiotic macrosphelide A, were examined in modified Czapek Dox broth (MCD). Antibiotic production was determined by incorporating heated (60 degrees C for 5 min) C. minitans spent culture filtrates of MCD (10%, v/v) into potato dextrose broth and assessing the ability of the filtrates to inhibit growth of S. sclerotiorum. All isolates grew over the temperature range of 10-30 degrees C, with the optimum at approximately 15-20 degrees C. Antibiotics were produced by all isolates at 10-30 degrees C. Culture filtrates of MCD from all isolates incorporated into PDB inhibited growth of S. sclerotiorum by > 50%, whereas there was a reduction in inhibition at 30 degrees C for Conio and IVT1 but not Contans. All three isolates grew over the pH range of 3-7, with greater biomass production in buffered pH 3-5 than the unbuffered control (pH 4.8) media. Antibiotics were produced by all isolates at pH 3-5. Culture filtrates of MCD from all three isolates grown at pH 3-5 inhibited growth of S. sclerotiorum, with the greatest effect on inhibition observed at pH 3. There were no differences in growth inhibition between isolates at pH 3 and 4, but culture filtrates from Conio grown at pH 5 inhibited S. sclerotiorum more than those of IVT1 grown at the same pH. The significance of these results for biocontrol and optimizing antibiotic production by C. minitans is discussed.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) | ||||
Journal or Publication Title: | Biocontrol Science and Technology | ||||
Publisher: | Taylor & Francis Ltd. | ||||
ISSN: | 0958-3157 | ||||
Official Date: | 2011 | ||||
Dates: |
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Volume: | Vol.21 | ||||
Number: | No.6 | ||||
Page Range: | pp. 721-731 | ||||
DOI: | 10.1080/09583157.2011.575211 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Biotechnology and Biological Sciences Research Council (BBSRC) |
Data sourced from Thomson Reuters' Web of Knowledge
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