Walley, Peter Glen, Carder, John, Skipper, Emma, Mathas, Evy, Lynn, James R. , Pink, David and Buchanan-Wollaston, Vicky. (2012) A new broccoli × broccoli immortal mapping population and framework genetic map: tools for breeders and complex trait analysis. Theoretical and Applied Genetics, Volume 124 (Number 3). pp. 467-484. ISSN 0040-5752

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Abstract

A unique broccoli x broccoli doubled haploid (DH) population has been created from the F-1 of a cross between two DH broccoli lines derived from cultivars Green Duke and Marathon. We genotyped 154 individuals from this population with simple sequence repeat and amplified fragment length polymorphism markers to create a B. oleracea L. var. italica 'intra-crop' specific framework linkage map. The map is composed of nine linkage groups with a total length of 946.7 cM. Previous published B. oleracea maps have been constructed using diverse crosses between morphotypes of B. oleracea; this map therefore represents a useful breeding resource for the dissection of broccoli specific traits. Phenotype data have been collected from the population over five growing seasons; the framework linkage map has been used to locate quantitative trait loci for agronomically important broccoli traits including head weight (saleable yield), head diameter, stalk diameter, weight loss and relative weight loss during storage, as well as traits for broccoli leaf architecture. This population and associated linkage map will aid breeders to directly map agronomically important traits for the improvement of elite broccoli cultivars.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Cole crops -- Genetics, Arabidopsis thaliana -- Genetics, Plant genomes, Gene mapping, Haploidy
Journal or Publication Title:	Theoretical and Applied Genetics
Publisher:	Springer
ISSN:	0040-5752
Date:	2012
Volume:	Volume 124
Number:	Number 3
Number of Pages:	18
Page Range:	pp. 467-484
Identification Number:	10.1007/s00122-011-1721-6
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Syngenta Seeds Ltd., Horticulture Research International (Great Britain), University of Birmingham
Grant number:	BB/E00671X/1 (BBSRC)
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URI:	http://wrap.warwick.ac.uk/id/eprint/42165

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