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GISP binding to TSG101 increases GABABreceptor stability by down-regulating ESCRT-mediated lysosomal degradation
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Kantamneni, Sriharsha, Holman, David, Wilkinson, Kevin A., Corrêa, Sônia A. L., Feligioni, Marco, Ogden, Simon, Fraser, William, Nishimune, Atsushi and Henley, Jeremy M. (2008) GISP binding to TSG101 increases GABABreceptor stability by down-regulating ESCRT-mediated lysosomal degradation. Journal of Neurochemistry, Vol.107 (No.1). pp. 86-95. doi:10.1111/j.1471-4159.2008.05580.x ISSN 0022-3042.
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Official URL: http://dx.doi.org/10.1111/j.1471-4159.2008.05580.x
Abstract
The neuron-specific G protein-coupled receptor interacting scaffold protein (GISP) is a multidomain, brain-specific protein derived from the A-kinase anchoring protein-9 gene. We originally isolated GISP as an interacting partner for the GABAB receptor subunit GABAB1. Here, we show that the protein tumour susceptibility gene 101 (TSG101), an integral component of the endosomal sorting machinery that targets membrane proteins for lysosomal degradation, also interacts with GISP. TSG101 co-immunoprecipitates with GISP from adult rat brain, and using GST pull-downs, we identified that the eighth coiled-coiled region of GISP is critical for TSG101 association. Intriguingly, although there is no direct interaction between GISP and the GABAB2 subunit, their co-expression in HEK293 cells increases levels of GABAB2. GISP also inhibits TSG101-dependent GABAB2 down-regulation in human embryonic kidney 293 cells whereas over-expression of a mutant GISP lacking the TSG101 binding domain has no effect on GABAB2 degradation. These data suggest that GISP can function as a negative regulator of TSG101-dependent lysosomal degradation of transmembrane proteins in neurons to promote receptor stability.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > Q Science (General) | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Journal of Neurochemistry | ||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
| ISSN: | 0022-3042 | ||||
| Official Date: | 2008 | ||||
| Dates: |
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| Volume: | Vol.107 | ||||
| Number: | No.1 | ||||
| Number of Pages: | 10 | ||||
| Page Range: | pp. 86-95 | ||||
| DOI: | 10.1111/j.1471-4159.2008.05580.x | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Wellcome Trust (WT), MRC , EU | ||||
| Grant number: | PL 005320 (EU) |
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