The Library

A SEPALLATA gene is involved in the development and ripening of strawberry (Fragaria xananassa Duch.) fruit, a non-climacteric tissue

Tools

Seymour, G. B. (Graham B.), Ryder, Carol D., Cevik, Volkan, Hammond, John P., Popovich, Alexandra, King , Graham J., Vrebalov, Julia, Giovannoni, James J. and Manning, Kenneth. (2010) A SEPALLATA gene is involved in the development and ripening of strawberry (Fragaria xananassa Duch.) fruit, a non-climacteric tissue. Journal of Experimental Botany, Vol.63 (No.2). 1179-1188 . ISSN 0022-0957

	PDF WRAP_Ryder_sepallata.pdf - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader Download (739Kb)
	PDF WRAP_ryder_coversheet.pdf - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader Download (38Kb)

Official URL: http://dx.doi.org/10.1093/jxb/erq360

Abstract

Climacteric and non-climacteric fruits have traditionally been viewed as representing two distinct programmes of ripening associated with differential respiration and ethylene hormone effects. In climacteric fruits, such as tomato and banana, the ripening process is marked by increased respiration and is induced and co-ordinated by ethylene, while in non-climacteric fruits, such as strawberry and grape, it is controlled by an ethylene-independent process with little change in respiration rate. The two contrasting mechanisms, however, both lead to texture, colour, and flavour changes that probably reflect some common programmes of regulatory control. It has been shown that a SEPALLATA(SEP)4-like gene is necessary for normal ripening in tomato. It has been demonstrated here that silencing a fruit-related SEP1/2-like (FaMADS9) gene in strawberry leads to the inhibition of normal development and ripening in the petal, achene, and receptacle tissues. In addition, analysis of transcriptome profiles reveals pleiotropic effects of FaMADS9 on fruit development and ripening-related gene expression. It is concluded that SEP genes play a central role in the developmental regulation of ripening in both climacteric and non-climacteric fruits. These findings provide important information to extend the molecular control of ripening in a non-climacteric fruit beyond the limited genetic and cultural options currently available.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Science > Life Sciences (2010- ) Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Strawberries -- Ripening, Strawberries -- Genetics
Journal or Publication Title:	Journal of Experimental Botany
Publisher:	Oxford University Press
ISSN:	0022-0957
Date:	29 November 2010
Volume:	Vol.63
Number:	No.2
Page Range:	1179-1188
Identification Number:	10.1093/jxb/erq360
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Great Britain. Dept. for Environment, Food & Rural Affairs (DEFRA)
References:	Alexander L, Grierson D. 2002. Ethylene biosynthesis and action in tomato: a model for climacteric fruit ripening. Journal of Experimental Botany 53, 2039–2055. Ampomah-Dwamena C, Morris BA, Sutherland P, Veit B, Yao JL. 2002. Down-regulation of TM29, a tomato SEPALLATA homolog, causes parthenocarpic fruit development and floral reversion. Plant Physiology 130, 605–617. Chang S, Puryear J, Cairney J. 1993. A simple and efficient method for isolating RNA from pine trees. Plant Molecular Biology Reporter 11, 113–116. Elitzur T, Vrebalov J, Giovannoni JJ, Goldschmidt EE, Friedman H. 2010. The regulation of MADS-box gene expression during ripening of banana and their regulatory interaction with ethylene. Journal of Experimental Botany 61, 1523–1535. Fei Z, Tang X, Alba RM, White RA, Ronning CM, Martin GB, Tanksley SD, Giovannoni JJ. 2004. Comprehensive EST analysis of tomato and comparative genomics of fruit ripening. The Plant Journal 40, 47–59. Ferra´ ndiz C. 2002. Regulation of fruit dehiscence in Arabidopsis. Journal of Experimental Botany 53, 2031–2038. Giovannoni JJ. 2004. Genetic regulation of fruit development and ripening. The Plant Cell 16, S170–S180. Given NK, Venis MA, Grierson D. 1988a. Hormonal regulation of ripening in the strawberry, a non-climacteric fruit. Planta 174, 402–406. Given NK, Venis MA, Grierson D. 1988b. Purification and properties of phenylalanine ammonia-lyase from strawberry fruit and its synthesis during ripening. Journal of Plant Physiology 133, 31–37. Hammond JP, Bowen HC, White PJ, Mills V, Pyke KA, Baker AJM, Whiting SN, May ST, Broadley MR. 2006. A comparison of the Thlaspicaerulescens and Thlaspiarvense shoot transcriptomes. New Phytologist 170, 239–260. Hammond JP, Broadley MR, Craigon DJ, Higgins J, Emmerson Z, Townsend H, White PJ, May ST. 2005. Using genomic DNA-based probe-selection to improve the sensitivity of high-density oligonucleotide arrays when applied to heterologous species. Plant Methods 1, 10. Hiscox JD, Israelstam GF. 1979. A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany 57, 1332–1334. Ito Y, Kitagawa M, Ihashi N, Yabe K, Kimbara J, Yasuda J, Ito H, Inakuma T, Hiroi S, Kasumi T. 2008. DNA-binding specificity, transcriptional activation potential, and the rin mutation effect for the tomato fruit-ripening regulator RIN. The Plant Journal 55,212–223. Judd WS, Campbell CS, Kellogg EA, Stevens PF. 1999. Plant systematics:a Phylogenetic approach. Sunderland: Mass:Sinauer Associates Inc. Malcomber ST, Kellogg EA. 2005. SEPALLATA gene diversification: brave new whorls. Trends in Plant Science 10, 427–435. Nitsch JP. 1950. Growth and morphogenesis of the strawberry as related to auxin. American Journal of Botany 37, 211–215. Palecanda L, Sharrock RA. 2001. Molecular and phenotypic specificity of an antisense PHYB gene in Arabidopsis. Plant Molecular Biology 46, 89–97. Robinson R, Tomes M. 1968. Ripening inhibitor: a gene with multiple effects on ripening. Report of the Tomato Genetics Cooperative 18, 36–37. Seymour GB, Taylor JE, Tucker GA. 1993. Biochemistry of fruit ripening. London, New York: Chapman & Hall. Van Engelen F, Molthoff J, Conner A, Nap J, Pereira A, Stiekema W. 1995. pBINPLUS: an improved plant transformation vector based on pBIN19. Transgenic Research 4, 288–290. Vrebalov J, Pan IL, Arroyo AJM, et al. 2009. Fleshy fruit expansion and ripening are regulated by the tomato SHATTERPROOF gene TAGL1. The Plant Cell 21, 3041–3062. Vrebalov J, Ruezinsky D, Padmanabhan V, White R, Medrano D, Drake R, Schuch W, Giovannoni JJ. 2002. A MADS-box gene necessary for fruit ripening at the tomato Ripening-Inhibitor (Rin) locus. Science 296, 343–346. Woolley LC, James DJ, Manning K. 2001. Purification and properties of an endo-b-1,4-glucanase from strawberry and downregulation of the corresponding gene, cel1. Planta 214, 11–21. Zahn LM, Kong H, Leebens-Mach JH, Kim S, Soltis PM, Landherr LL, Soltis DE, DePamphilis CW, Ma H. 2005. The evolution of the SEPALLATA subfamily of MADS-box genes: a preangiosperm origin with multiple duplications throughout angiosperm history. Genetics 169, 2209–2223.
URI:	http://wrap.warwick.ac.uk/id/eprint/427