The Library
Adenoviruses: Molecular Biology
Tools
Leppard, Keith (2008) Adenoviruses: Molecular Biology. In: Mahy, Brian W. J. and Van Regenmortel, Marc H. V., (eds.) Encyclopedia of Virology. Amsterdam ; London: Elsevier Ltd., pp. 17-23. ISBN 9780123739360
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1016/B978-012374410-4.00356-3
Abstract
The molecular biology of adenoviruses has been studied mainly using two human serotypes, 2 and 5. The virions are nonenveloped, icosahedral particles that contain a linear, double-stranded DNA genome of approximately 36 000 bp. Protein fibers project from the vertices of the particle, and these mediate primary attachment to the target cell. Cell entry is via receptor-mediated endocytosis. Following escape from the endosome, the partially uncoated capsid is transported to the nuclear surface where the DNA enters the nucleus. Transcription of mRNAs occurs in temporal phases, using host RNA polymerase II. Proteins produced early after infection modify the host environment, including mitigating host antiviral responses, and provide essential DNA replication functions, including DNA polymerase. Once replication has begun, high levels of late proteins are made, from which progeny particles are assembled. Detailed mechanisms of particle release have not been determined. In established cell lines, this cycle takes approximately 24 h, and considerably longer in primary cells. The detailed understanding of adenovirus replication is being used to develop this virus as a gene delivery vector.
Copyright © 2008 Elsevier Ltd. All rights reserved.
Item Type: | Book Item | ||||
---|---|---|---|---|---|
Subjects: | Q Science > Q Science (General) | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Adenoviridae: molecular biology | ||||
Publisher: | Elsevier Ltd. | ||||
Place of Publication: | Amsterdam ; London | ||||
ISBN: | 9780123739360 | ||||
Book Title: | Encyclopedia of Virology | ||||
Editor: | Mahy, Brian W. J. and Van Regenmortel, Marc H. V. | ||||
Official Date: | 2008 | ||||
Dates: |
|
||||
Volume: | Vol.1 | ||||
Number of Pages: | 7 | ||||
Page Range: | pp. 17-23 | ||||
DOI: | 10.1016/B978-012374410-4.00356-3 | ||||
Status: | Not Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |