Byrne-Bailey, K. G., Gaze, William H., Kay, Paul, Boxall, Alistair B.A. , Hawkey, Peter M. and Wellington, E. M. H. (Elizabeth M. H.), 1954-. (2009) Prevalence of sulfonamide resistance genes in bacterial isolates from manured agricultural soils and pig slurry in the United Kingdom. Antimicrobial Agents and Chemotherapy, Vol.53 (No.2). pp. 696-702. ISSN 0066-4804

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Abstract

Prevalence of three sulfonamide resistance genes, sul1, sul2 and sul3 and sulfachloropyridazine (SCP) resistance was determined in bacteria isolated from UK manured agricultural clay soils and slurry samples, over a two year period. Slurry from tylosin-fed pigs amended with SCP and oxytetracycline (OTC) was used for manuring. Sul gene positive isolates were further screened for the presence of class 1 and 2 integrons. Phenotypic resistance to SCP was significantly higher in pig slurry and post application soil than in pre-application soil. Of 5isolates, 23 % carried sul1, 18 % sul2 and 9 % sul3 only. Two percent of isolates contained all three sul genes. Class 1 and class 2 integrons were identified in 5 % and 11.7 % of sul positive isolates. In previous reports, sul1 was linked to class 1 integrons, but in this study only 8 % of sul1 positive isolates carried the intI1 gene. Sulfonamide resistant pathogens were identified in slurry amended soil and soil leachate, including Shigella flexneri, Aerococcus spp. and Acinetobacter baumanni, suggesting a potential environmental reservoir. Sulfonamide resistance in Psychrobacter, Enterococcus and Bacillus spp. is reported for the first time, and this study also provides the first description of the genotype sul1, sul2 and sul3 outside the Enterobacteriacae, and in the soil environment.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	Sulfonamides, Drug resistance, Soil microbiology, Bacterial genetics, Bacteriology, Agricultural
Journal or Publication Title:	Antimicrobial Agents and Chemotherapy
Publisher:	American Society for Microbiology
ISSN:	0066-4804
Date:	February 2009
Volume:	Vol.53
Number:	No.2
Page Range:	pp. 696-702
Identification Number:	10.1128/AAC.00652-07
Status:	Peer Reviewed
Access rights to Published version:	Restricted or Subscription Access
Funder:	Natural Environment Research Council (Great Britain) (NERC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Wyeth Pharmaceutical Company
Grant number:	NER/A/S/2000/01253 (NERC)
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URI:	http://wrap.warwick.ac.uk/id/eprint/4280

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