The Library

Integron prevalence and diversity in manured soil

Tools

Byrne-Bailey, K. G., Gaze, William H., Zhang, Lihong, Kay, Paul, Boxall, Alistair B.A. , Hawkey, P. M. and Wellington, E. M. H.. (2011) Integron prevalence and diversity in manured soil. Applied and Environmental Microbiology, Vol.77 (No.2). pp. 684-687. ISSN 0099-2240

PDF
WRAP_Wellington_0380313-lf-150211-byrne-bailey2010.pdf - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (168Kb)

Official URL: http://dx.doi.org/10.1128/AEM.01425-10

Abstract

Integron abundance and diversity were studied in soil amended with pig slurry. Real-time PCR illustrated a significant increase in class 1 integron prevalence post slurry-application with increased prevalence still evident at 10 months post-application. Culture dependent data revealed 10 genera, including putative human pathogens, carrying class 1 and 2 integrons.

Item Type:

Journal Article

Subjects:

Q Science > QR Microbiology

Divisions:

Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Faculty of Science > Life Sciences (2010- )

Library of Congress Subject Headings (LCSH):

Drug resistance, Bacteriology, Agricultural, Soil microbiology, Swine -- Manure

Journal or Publication Title:

Applied and Environmental Microbiology

Publisher:

American Society for Microbiology

ISSN:

0099-2240

Official Date:

January 2011

Dates:

Date	Event
January 2011	Published

Volume:

Vol.77

Number:

No.2

Page Range:

pp. 684-687

Identification Number:

10.1128/AEM.01425-10

Status:

Peer Reviewed

Access rights to Published version:

Open Access

Funder:

Natural Environment Research Council (Great Britain) (NERC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Wyeth Pharmaceutical Company

Grant number:

NER/A/S/2000/01253 (NERC)

References:

1. Acinas, S. G., L. A. Marcelino, V. Klepac-Ceraj, and M. F. Polz. 2004. Divergence and redundancy of 16S rRNA sequences in genomes with multiple rrn operons. J Bacteriol 186:2629-35.
2. Agerso, Y., and D. Sandvang. 2005. Class 1 integrons and tetracycline resistance genes in alcaligenes, arthrobacter, and Pseudomonas spp. isolated from pigsties and manured soil. Appl Environ Microbiol 71:7941-7.
3. Binh, C. T., H. Heuer, M. Kaupenjohann, and K. Smalla. 2009. Diverse aadA gene cassettes on class 1 integrons introduced into soil via spread manure. Res Microbiol.
4. Blackwell, P. A., A. B. Boxall, P. Kay, and H. Noble. 2005. Evaluation of a lower tier exposure assessment model for veterinary medicines. J Agric Food Chem 53:2192-201.
5. Boxall, A. B., P. Blackwell, R. Cavallo, P. Kay, and J. Tolls. 2002. The sorption and transport of a sulphonamide antibiotic in soil systems. Toxicol Lett 131:19-28.
6. Byrne-Bailey, K. G., W. H. Gaze, P. Kay, A. B. Boxall, P. M. Hawkey, and E. M. Wellington. 2009. Prevalence of sulfonamide resistance genes in bacterial isolates from manured agricultural soils and pig slurry in the United Kingdom. Antimicrob Agents Chemother 53:696-702.
7. Collis, C. M., and R. M. Hall. 1995. Expression of antibiotic resistance genes in the integrated cassettes of integrons. Antimicrob Agents Chemother 39:155-62.
8. Fluit, A. C., and F. J. Schmitz. 2004. Resistance integrons and super-integrons. Clin Microbiol Infect 10:272-88.
9. Gaze, W. H., N. Abdouslam, P. M. Hawkey, and E. M. Wellington. 2005. Incidence of class 1 integrons in a quaternary ammonium compound-polluted environment. Antimicrob Agents Chemother 49:1802-7.
10. Gillings, M. R., D. Xuejun, S. A. Hardwick, M. P. Holley, and H. W. Stokes. 2009. Gene cassettes encoding resistance to quaternary ammonium compounds: a role in the origin of clinical class 1 integrons? Isme J 3:209-15.
11. Heuer, H., and K. Smalla. 2007. Manure and sulfadiazine synergistically increased bacterial antibiotic resistance in soil over at least two months. Environmental Microbiology 9:657.
12. Kay, P., P. A. Blackwell, and A. B. Boxall. 2004. Fate of veterinary antibiotics in a macroporous tile drained clay soil. Environ Toxicol Chem 23:1136-44.
13. Ku, S. C., P. R. Hsueh, P. C. Yang, and K. T. Luh. 2000. Clinical and microbiological characteristics of bacteremia caused by Acinetobacter lwoffii. Eur J Clin Microbiol Infect Dis 19:501-5.
14. Lapierre, L., J. Cornejo, C. Borie, C. Toro, and B. San Martin. 2008. Genetic characterization of antibiotic resistance genes linked to class 1 and class 2 integrons in commensal strains of Escherichia coli isolated from poultry and swine. Microb Drug Resist 14:265-72.
15. Martin, V., A. I. Vela, M. Gilbert, J. Cebolla, J. Goyache, L. Dominguez, and J. F. Fernandez-Garayzabal. 2007. Characterization of Aerococcus viridans isolates from swine clinical specimens. J Clin Microbiol 45:3053-7.
16. Nandi, S., J. J. Maurer, C. Hofacre, and A. O. Summers. 2004. Gram-positive bacteria are a major reservoir of Class 1 antibiotic resistance integrons in poultry litter. Proc Natl Acad Sci U S A 101:7118-22.
17. Paulsen, I. T., T. G. Littlejohn, P. Radstrom, L. Sundstrom, O. Skold, G. Swedberg, and R. A. Skurray. 1993. The 3' conserved segment of integrons contains a gene associated with multidrug resistance to antiseptics and disinfectants. Antimicrob Agents Chemother 37:761-8.
18. Rosser, S. J., and H. K. Young. 1999. Identification and characterization of class 1 integrons in bacteria from an aquatic environment. J Antimicrob Chemother 44:11-8.
19. Stokes, H. W., C. L. Nesbo, M. Holley, M. I. Bahl, M. R. Gillings, and Y. Boucher. 2006. Class 1 integrons potentially predating the association with tn402-like transposition genes are present in a sediment microbial community. J Bacteriol 188:5722-30.
20. Tauch, A., S. Gotker, A. Puhler, J. Kalinowski, and G. Thierbach. 2002. The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100. Plasmid 48:117-29.
21. Thorsted, P. B., D. P. Macartney, P. Akhtar, A. S. Haines, N. Ali, P. Davidson, T. Stafford, M. J. Pocklington, W. Pansegrau, B. M. Wilkins, E. Lanka, and C. M. Thomas. 1998. Complete sequence of the IncPbeta plasmid R751: implications for evolution and organisation of the IncP backbone. J Mol Biol 282:969-90.
22. White, P. A., C. J. McIver, and W. D. Rawlinson. 2001. Integrons and gene cassettes in the enterobacteriaceae. Antimicrob Agents Chemother 45:2658-61.