The Library
Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins
Tools
Tools
Tools
Cridge, A. G., Castelli, L. M., Smirnova, J. B., Selley, J. N., Rowe, W., Hubbard, S. J., McCarthy, John E. G., Ashe, M. P., Grant, C. M. and Pavitt, G. D. (2010) Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins. Nucleic Acids Research, Vol.38 (No.22). pp. 8039-8050. doi:10.1093/nar/gkq686
Research output not available from this repository, contact author.
Official URL: http://dx.doi.org/10.1093/nar/gkq686
Abstract
eIF4E-binding proteins (4E-BPs) regulate translation of mRNAs in eukaryotes. However the extent to which specific mRNA targets are regulated by 4E-BPs remains unknown. We performed translational profiling by microarray analysis of polysome and monosome associated mRNAs in wild-type and mutant cells to identify mRNAs in yeast regulated by the 4E-BPs Caf20p and Eap1p; the first-global comparison of 4E-BP target mRNAs. We find that yeast 4E-BPs modulate the translation of >1000 genes. Most target mRNAs differ between the 4E-BPs revealing mRNA specificity for translational control by each 4E-BP. This is supported by observations that eap1Δ and caf20Δ cells have different nitrogen source utilization defects, implying different mRNA targets. To account for the mRNA specificity shown by each 4E-BP, we found correlations between our data sets and previously determined targets of yeast mRNA-binding proteins. We used affinity chromatography experiments to uncover specific RNA-stabilized complexes formed between Caf20p and Puf4p/Puf5p and between Eap1p and Puf1p/Puf2p. Thus the combined action of each 4E-BP with specific 3′-UTR-binding proteins mediates mRNA-specific translational control in yeast, showing that this form of translational control is more widely employed than previously thought.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Nucleic Acids Research | ||||
| Publisher: | Oxford University Press | ||||
| ISSN: | 0305-1048 | ||||
| Official Date: | 2010 | ||||
| Dates: |
|
||||
| Volume: | Vol.38 | ||||
| Number: | No.22 | ||||
| Page Range: | pp. 8039-8050 | ||||
| DOI: | 10.1093/nar/gkq686 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
