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Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins
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Cridge, A. G., Castelli, L. M., Smirnova, J. B., Selley, J. N., Rowe, W., Hubbard, S. J., McCarthy, John E. G., Ashe, M. P., Grant, C. M. and Pavitt, G. D. (2010) Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins. Nucleic Acids Research, Vol.38 (No.22). pp. 8039-8050. doi:10.1093/nar/gkq686
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Official URL: http://dx.doi.org/10.1093/nar/gkq686
Abstract
eIF4E-binding proteins (4E-BPs) regulate translation of mRNAs in eukaryotes. However the extent to which specific mRNA targets are regulated by 4E-BPs remains unknown. We performed translational profiling by microarray analysis of polysome and monosome associated mRNAs in wild-type and mutant cells to identify mRNAs in yeast regulated by the 4E-BPs Caf20p and Eap1p; the first-global comparison of 4E-BP target mRNAs. We find that yeast 4E-BPs modulate the translation of >1000 genes. Most target mRNAs differ between the 4E-BPs revealing mRNA specificity for translational control by each 4E-BP. This is supported by observations that eap1Δ and caf20Δ cells have different nitrogen source utilization defects, implying different mRNA targets. To account for the mRNA specificity shown by each 4E-BP, we found correlations between our data sets and previously determined targets of yeast mRNA-binding proteins. We used affinity chromatography experiments to uncover specific RNA-stabilized complexes formed between Caf20p and Puf4p/Puf5p and between Eap1p and Puf1p/Puf2p. Thus the combined action of each 4E-BP with specific 3′-UTR-binding proteins mediates mRNA-specific translational control in yeast, showing that this form of translational control is more widely employed than previously thought.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Nucleic Acids Research | ||||
| Publisher: | Oxford University Press | ||||
| ISSN: | 0305-1048 | ||||
| Official Date: | 2010 | ||||
| Dates: |
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| Volume: | Vol.38 | ||||
| Number: | No.22 | ||||
| Page Range: | pp. 8039-8050 | ||||
| DOI: | 10.1093/nar/gkq686 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published |
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