Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart
Inagawa, T., Yamada-Inagawa, T., Eydmann, T., Mian, I. S., Wang, T. S. and Dalgaard, Jacob Z.. (2009) Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart. Proceedings of the National Academy of Sciences of the United States of America, Vol.106 (No.19). pp. 7927-7932. ISSN 0027-8424Full text not available from this repository.
Official URL: http://dx.doi.org/10.1073/pnas.0812323106
Here, we identify a phylogenetically conserved Schizosaccharomyces pombe factor, named Rtf2, as a key requirement for efficient replication termination at the site-specific replication barrier RTS1. We show that Rtf2, a proliferating cell nuclear antigen-interacting protein, promotes termination at RTS1 by preventing replication restart; in the absence of Rtf2, we observe the establishment of “slow-moving” Srs2-dependent replication forks. Analysis of the pmt3 (SUMO) and rtf2 mutants establishes that pmt3 causes a reduction in RTS1 barrier activity, that rtf2 and pmt3 are nonadditive, and that pmt3 (SUMO) partly suppresses the rtf2-dependent replication restart. Our results are consistent with a model in which Rtf2 stabilizes the replication fork stalled at RTS1 until completion of DNA synthesis by a converging replication fork initiated at a flanking origin.
|Item Type:||Journal Article|
|Divisions:||Faculty of Medicine > Warwick Medical School > Biomedical Cell Biology
Faculty of Medicine > Warwick Medical School
|Journal or Publication Title:||Proceedings of the National Academy of Sciences of the United States of America|
|Publisher:||National Academy of Sciences|
|Date:||12 May 2009|
|Page Range:||pp. 7927-7932|
|Access rights to Published version:||Restricted or Subscription Access|
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