Eydmann, T., Sommariva, E., Inagawa, T., Mian, S., Klar, A. J. S. and Dalgaard, Jacob Z.. (2008) Rtf1-mediated eukaryotic site-specific replication termination. Genetics, Vol.180 (No.1). pp. 27-39. ISSN 0016-6731

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Abstract

The molecular mechanisms mediating eukaryotic replication termination and pausing remain largely unknown. Here we present the molecular characterization of Rtf1 that mediates site-specific replication termination at the polar Schizosaccharomyces pombe barrier RTS1. We show that Rtf1 possesses two chimeric myb/SANT domains: one is able to interact with the repeated motifs encoded by the RTS1 element as well as the elements enhancer region, while the other shows only a weak DNA binding activity. In addition we show that the C-terminal tail of Rtf1 mediates self-interaction, and deletion of this tail has a dominant phenotype. Finally, we identify a point mutation in Rtf1 domain I that converts the RTS1 element into a replication barrier of the opposite polarity. Together our data establish that multiple protein DNA and protein–protein interactions between Rtf1 molecules and both the repeated motifs and the enhancer region of RTS1 are required for site-specific termination at the RTS1 element.

Item Type:	Journal Article
Divisions:	Faculty of Medicine > Warwick Medical School > Biomedical Cell Biology Faculty of Medicine > Warwick Medical School
Journal or Publication Title:	Genetics
Publisher:	Genetics Society of America
ISSN:	0016-6731
Date:	September 2008
Volume:	Vol.180
Number:	No.1
Page Range:	pp. 27-39
Identification Number:	10.1534/genetics.108.089243
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
URI:	http://wrap.warwick.ac.uk/id/eprint/43969

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