Buttrick, G. J., Lancaster, T. C., Meadows, J. C. and Millar, Jonathan B. A.. (2012) Plo1 phosphorylates Dam1 to promote chromosome bi-orientation in fission yeast. Journal of Cell Science, Vol.125 (No.7). pp. 1645-1651. ISSN 0021-9533

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Abstract

The fungal specific heterodecameric outer kinetochore DASH complex facilitates the interaction of kinetochores with spindle microtubules. In budding yeast, where kinetochores bind a single microtubule, the DASH complex is essential and phosphorylation of Dam1 by the Aurora kinase homologue, Ipl1, causes detachment of kinetochores from spindle microtubules. We demonstrate that in the distantly related fission yeast, where the DASH complex is not essential for viability and kinetochores bind multiple microtubules, Dam1 is instead phosphorylated on serine 143 by the Polo kinase homologue, Plo1, during prometaphase and metaphase. This phosphorylation site is conserved in most fungal Dam1 proteins, including budding yeast Dam1. We show Dam1 phosphorylation by Plo1 is dispensable for DASH assembly and chromosome retrieval but instead aids tension-dependent chromosome bi-orientation.

Item Type:	Journal Article
Divisions:	Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Medicine > Warwick Medical School
Journal or Publication Title:	Journal of Cell Science
Publisher:	The Company of Biologists Ltd.
ISSN:	0021-9533
Official Date:	2012
Dates:	Date Event 2012 Published
Volume:	Vol.125
Number:	No.7
Page Range:	pp. 1645-1651
Identification Number:	10.1242/jcs.096826
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
URI:	http://wrap.warwick.ac.uk/id/eprint/44048

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