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Environmental monitoring of Mycobacterium bovis in badger feces and badger sett soil by real-time PCR, as confirmed by immunofluorescence, immunocapture, and cultivation

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Sweeney, F. P., Courtenay, Orin, Hibberd, V. (Victoria), Hewinson, R. G., Reilly, L. A., Gaze, William H. and Wellington, E. M. H. (Elizabeth M. H.), 1954-. (2007) Environmental monitoring of Mycobacterium bovis in badger feces and badger sett soil by real-time PCR, as confirmed by immunofluorescence, immunocapture, and cultivation. Applied and Environmental Microbiology, Vol.73 (No.22). pp. 7471-7473. ISSN 0099-2240

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Official URL: http://dx.doi.org/10.1128/AEM.00978-07

Abstract

Real-time PCR was used to detect and quantify Mycobacterium bovis cells in naturally infected soil and badger faeces. Immunomagnetic capture, immunofluorescence and selective culture confirmed species identification and cell viability. These techniques will prove useful for monitoring M. bovis in the environment and for elucidating transmission routes between wildlife and cattle.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH):	Mycobacterium bovis, Polymerase chain reaction, Badgers -- Infections
Journal or Publication Title:	Applied and Environmental Microbiology
Publisher:	American Society for Microbiology
ISSN:	0099-2240
Date:	November 2007
Volume:	Vol.73
Number:	No.22
Page Range:	pp. 7471-7473
Identification Number:	10.1128/AEM.00978-07
Status:	Peer Reviewed
Access rights to Published version:	Open Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)
Grant number:	BBS/B/08868 (BBSRC)
References:	1. Brosch R., S.V. Gordon, M. Marmiesse, P. Brodin, C. Buchrieser, K. Eiglmeier, T. Garnier, C. Gutierrez, R.G. Hewinson, K. Kremer, L. M. Parsons, A. S. Pym, S. Samper, D. van Soolingen, and S. T. Cole. 2002. A new evolutionary scenario for the Mycobacterium tuberculosis complex. PNAS 99:3684-3689. 2. Courtenay, O., L.A. Reilly, F.P. Sweeney, V. Hibberd, S. Bryan, A. Ul-Hassan, C. Newman, D.W. Macdonald, R.J. Delahay, G.J. Wilson and E.M.H. Wellington. 2006. Is Mycobacterium bovis in the environment important for the persistence of bovine tuberculosis? Biol. Lett. 2:460-462. 3. Francis, J. 1947. Bovine tuberculosis: including a contrast with human tuberculosis, Staples Press, London, p92. 4. Gallagher, J. and R.S. Clifton-Hadley. 2000. Tuberculosis in badgers: a review of the disease and its significance for other animals. Res. Vet. Sci. 69:203-217. 5. Garnier, T., K. Eiglmeier, J.C. Camus, N. Medina, H. Mansoor, M. Pryor, S. Duthoy, S. Grondin, C. Lacroix, C. Monsempe, S. Simon, B. Harris, R. Atkin, J. Doggett, R. Mayes, L. Keating, P.R. Wheeler, J. Parkhill, B.G. Barrell, S.T. Cole, S.V. Gordon and R.G. Hewinson. 2005. The complete DNA sequence of Mycobacterium bovis. PNAS 45:1113-1244. 6. Goodger J., A. Nolan, W.P. Russell, D.J. Dalley, C.J. Thorns, F.A. Stuart, P. Croston and D.G. Newell. 1994. Serodiagnosis of Mycobacterium bovis infection in badgers: development of an indirect ELISA using a 25 kDa antigen. Vet. Rec. 135:82-85. 7. Guan, T.Y. and R.A. Holley. 2003. Pathogen survival in swine manure environments and transmission of human enteric illness – a review. J. Environ. Qual. 32:383-392. 8. Hewinson, R.G., S.L. Michell, W.P. Russell, R.A. McAdam and W.R. Jacobs Jr. 1996. Molecular characterisation of MPT83: a seroactive antigen of Mycobacterium tuberculosis with homology to MPT70. Scand. J. Immunol. 43:490-499. 9. Nolan, A. and J.W. Wilesmith. 1994. Tuberculosis in badgers (Meles meles). Vet. Microbiol. 28:103-109. 10. O’Reilly, L.M. and C.J. Daborn. 1995. The epidemiology of Mycobacterium bovis infections in animals and man: a review. Tuber. Lung Dis. 76 (S1):1-46. 11. Palmer, M.V., W.R. Waters and D.L . Whipple. 2003. Aerosol exposure of virulent Mycobacterium bovis to cattle. Tuberculosis 82:275-282. 12. Primm, T.P., C.A. Lucero and J.O. Falkinham 3rd. 2004. Health impacts of environmental mycobacteria. Clin. Microbiol. Rev. 17:98-106. 13. Sweeney, F.P., O. Courtenay, A. Ul-Hassan, V. Hibberd, L.A. Reilly and E.M.H. Wellington. 2006. Immunomagnetic recovery of Mycobacterium bovis from naturally infected environmental samples. Lett. Appl. Microbiol. 2:460-462. 14. Wiker, H.G., S. Nagai, R.G. Hewinson, W.P. Russell and M. Harboe. 1996. Heterogenous expression of the related MPB70 and MPB83 proteins distinguish various substrains of Mycobacterium bovis BCG and Mycobacterium tuberculosis H37Rv. Scand. J. Immunol. 43:374-80. 15. Wiker, H.G., K.P. Lyashchenko, A.M. Aksoy, K.A. Lightbody, J.M. Pollock, S.V. Komissarenko, S.O. Bobrovnik, I.N. Kolesnikova, L.O. Mykhalsky, M.L. Gennaro and M. Harboe. 1998. Immunochemical characterization of the MPB70/80 and MPB83 proteins of Mycobacterium bovis. Infect. Immun. 66:1445-52. 16. Young, J.S., E. Gormley and E.M.H. Wellington. 2005. Molecular detection of Mycobacterium bovis and Mycobacterium bovis BCG (Pasteur) in soil. Appl. Environ. Microbiol 71:1946-1952
URI:	http://wrap.warwick.ac.uk/id/eprint/4414