Tandem mass spectrometry of polymeric materials
Jackson, Anthony Trevor (1995) Tandem mass spectrometry of polymeric materials. PhD thesis, University of Warwick.
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Mass spectrometry and tandem mass spectrometry (MS/MS) has been employed to
analyse peptides (<600 daltons), synthetic polymers of low molecular weight (<10,000
daltons) and a mixture of polymer additives (300-1200 daltons). Mass spectrometry
experiments were performed on a four sector mass spectrometer, a tandem quadrupole
mass spectrometer and a time-of-flight instrument. High and low energy collision
induced dissociation (CID) spectra were obtained by means of a four sector mass
spectrometer and a tandem quadrupole instrument respectively. Surface induced
dissociation (SID) spectra of peptides were obtained by means of a four sector mass
spectrometer with a modified collision cell in the third field free region.
Sequence data were generated by SID from protonated and cationated precursor ions
of all four peptides analysed. Furthermore, broad metastable ion peaks were observed
in the spectra, arising from fragmentation of precursor ions in the field free region
between the electric sector and the magnetic sector of the second mass spectrometer.
Field desorption was a good ionisation technique for the generation of molecular
weight information from the polymer additives used. High energy eID was found to
be more applicable than low energy eID to the structural determination of polymer
additives as characteristic ions were observed in the spectra. Mechanisms have been
proposed for the generation of some of the fragment ions observed.
Ultraviolet-matrix assisted laser desorptionlionisation spectra of synthetic polymers of
low molecular weight (<10,000 daltons) were used to calculate molecular weight
averages. Furthermore, end group information was also obtained from CID spectra of
the same polymer samples.
End group and structural information was also obtained from polystyrene and a
substituted polystyrene by means of FD-MS/MS.
|Item Type:||Thesis or Dissertation (PhD)|
|Subjects:||Q Science > QD Chemistry|
|Library of Congress Subject Headings (LCSH):||Mass spectrometry, Tandem mass spectrometry, Peptides -- Spectra, Polymers -- Spectra, Polymers -- Additives -- Spectra|
|Official Date:||November 1995|
|Institution:||University of Warwick|
|Theses Department:||Department of Chemistry|
|Supervisor(s)/Advisor:||Jennings, Keith R. ; Scrivens, James|
|Sponsors:||Engineering and Physical Sciences Research Council (EPSRC) ; Imperial Chemical Industries, ltd. ; British Mass Spectrometry Society|
|Extent:||xxxiv, 302 p.|
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