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Laticifer-specific cis-prenyltransferase silencing affects the rubber, triterpene, and inulin content of taraxacum brevicorniculatum
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Post, J., van Deenen, N., Fricke, J., Kowalski, N., Wurbs, D., Schaller, H., Eisenreich, W., Huber, C., Twyman, Richard M., Prufer, D. and Gronover, C. S. (2012) Laticifer-specific cis-prenyltransferase silencing affects the rubber, triterpene, and inulin content of taraxacum brevicorniculatum. Plant Physiology, Vol.158 (No.3). pp. 1406-1417. doi:10.1104/pp.111.187880
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Official URL: http://dx.doi.org/10.1104/pp.111.187880
Abstract
Certain Taraxacum species, such as Taraxacum koksaghyz and Taraxacum brevicorniculatum, produce large amounts of high-quality natural rubber in their latex, the milky cytoplasm of specialized cells known as laticifers. This high-molecular mass biopolymer consists mainly of poly(cis-1,4-isoprene) and is deposited in rubber particles by particle-bound enzymes that carry out the stereospecific condensation of isopentenyl diphosphate units. The polymer configuration suggests that the chain-elongating enzyme (rubber transferase; EC 2.5.1.20) is a cis-prenyltransferase (CPT). Here, we present a comprehensive analysis of transgenic T. brevicorniculatum plants in which the expression of three recently isolated CPTs known to be associated with rubber particles (TbCPT1 to -3) was heavily depleted by laticifer-specific RNA interference (RNAi). Analysis of the CPT-RNAi plants by nuclear magnetic resonance, size-exclusion chromatography, and gas chromatography-mass spectrometry indicated a significant reduction in rubber biosynthesis and a corresponding 50% increase in the levels of triterpenes and the main storage carbohydrate, inulin. Transmission electron microscopy revealed that the laticifers in CPT-RNAi plants contained fewer and smaller rubber particles than wild-type laticifers. We also observed lower activity of hydroxymethylglutaryl-coenzyme A reductase, the key enzyme in the mevalonate pathway, reflecting homeostatic control of the isopentenyl diphosphate pool. To our knowledge, this is the first in planta demonstration of latex-specific CPT activity in rubber biosynthesis. © 2012 American Society of Plant Biologists.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > Q Science (General) | ||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
| Journal or Publication Title: | Plant Physiology | ||||
| Publisher: | American Society of Plant Biologists | ||||
| ISSN: | 0032-0889 | ||||
| Official Date: | March 2012 | ||||
| Dates: |
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| Volume: | Vol.158 | ||||
| Number: | No.3 | ||||
| Page Range: | pp. 1406-1417 | ||||
| DOI: | 10.1104/pp.111.187880 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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