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Biochemical characterization of the Mycobacterium tuberculosis phosphoribosyl-1-pyrophosphate synthetase
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Alderwick, Luke J., Lloyd, Georgina S., Lloyd, Adrian, Lovering, Andrew L., Eggeling, L. and Besra, Gurdyal S. (2011) Biochemical characterization of the Mycobacterium tuberculosis phosphoribosyl-1-pyrophosphate synthetase. Glycobiology, Vol.21 (No.4). pp. 410-425. doi:10.1093/glycob/cwq173
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Official URL: http://dx.doi.org/10.1093/glycob/cwq173
Abstract
Mycobacterium tuberculosis arabinogalactan (AG) is an essential cell wall component. It
provides a molecular framework serving to connect peptidoglycan to the outer mycolic
acid layer. The biosynthesis of the arabinan domains of AG and lipoarabinomannan
(LAM), occurs via a combination of membrane bound arabinofuranosyltransferases, all
of which utilise decaprenol-1-monophosphorabinose as a substrate (DPA). The source of
arabinose ultimately destined for deposition into cell wall AG or LAM, originates
exclusively from phosphoribosyl-1-pyrophosphate (pRpp), a central metabolite which is
also required for other essential metabolic processes, such as de novo purine and
pyrimidne biosynthesis. In M. tuberculosis, a single pRpp synthetase enzyme (Mt-PrsA)
is soley responsible for the generation of pRpp, by catalysing the transfer of
pyrophosphate from ATP to the C1 hydroxyl position of ribose-5-phosphate. Here, we
report a detailed biochemical and biophysical study of Mt-PrsA, which exhibits the most
rapid enzyme kinetics reported for a pRpp synthetase.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QR Microbiology | ||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010) Faculty of Science > Life Sciences (2010- ) |
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| Library of Congress Subject Headings (LCSH): | Mycobacterium tuberculosis, Arabinogalactan -- Synthesis, Metabolites -- Synthesis, Ligases -- Analysis | ||||
| Journal or Publication Title: | Glycobiology | ||||
| Publisher: | Oxford University Press | ||||
| ISSN: | 1460-2423 | ||||
| Official Date: | April 2011 | ||||
| Dates: |
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| Volume: | Vol.21 | ||||
| Number: | No.4 | ||||
| Page Range: | pp. 410-425 | ||||
| DOI: | 10.1093/glycob/cwq173 | ||||
| Status: | Peer Reviewed | ||||
| Access rights to Published version: | Open Access | ||||
| Funder: | Royal Society (Great Britain), Lister Institute of Preventive Medicine, Medical Research Council (Great Britain) (MRC), Wellcome Trust (London, England) | ||||
| Grant number: | 081569/Z/06/Z (Wellcome) |
Data sourced from Thomson Reuters' Web of Knowledge
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